Publication: Characterization of recombinant dengue-2 virus derived from a single nucleotide substitution in the 5 ′ noncoding region
Issued Date
2010-05-06
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ISSN
11107251
11107243
11107243
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2-s2.0-77951672953
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Biomedicine and Biotechnology. Vol.2010, (2010)
Suggested Citation
Vijittra Leardkamolkarn, Wipawan Sirigulpanit, Richard M. Kinney Characterization of recombinant dengue-2 virus derived from a single nucleotide substitution in the 5 ′ noncoding region. Journal of Biomedicine and Biotechnology. Vol.2010, (2010). doi:10.1155/2010/934694 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28715
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Title
Characterization of recombinant dengue-2 virus derived from a single nucleotide substitution in the 5 ′ noncoding region
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Abstract
Variants of wild-type dengue serotype 2 (DEN-2) virus containing nucleotide substitutions at positions 14, 15, or 57 in the 5 ′ NCR were constructed by PCR-mediated site-directed mutagenesis. All three viruses containing a single point substitution demonstrated attenuation phenotype as evidenced by decreases replication and plaque size in cell culture assay. All three variants were less neurovirulent in newborn mice compared to the wild type. The mutants were immunogenic in adult mice immunogenicity and maintained stable replication characteristics following passage in mice. The variant viruses were competent for replication in Aedes aegypi mosquito vector, albeit at lower levels of infection and dissemination in the mosquito than the wild-type Den-2 16681 virus. Although all of the viruses, including the wild type, were found transmissible in mosquito life cycles, they were found subsequentially decreased in efficiency of infection, transmission, and dissemination rates along the mosquito generations and all of them remained genetically stable. © 2010 Vijittra Leardkamolkarn et al.