Publication: The autoregulator receptor homologue AvaR3 plays a regulatory role in antibiotic production, mycelial aggregation and colony development of Streptomyces avermitilis
1
Issued Date
2011-08-01
Resource Type
ISSN
13500872
Other identifier(s)
2-s2.0-79961061778
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microbiology. Vol.157, No.8 (2011), 2266-2275
Suggested Citation
Kiyoko T. Miyamoto, Shigeru Kitani, Mamoru Komatsu, Haruo Ikeda, Takuya Nihira The autoregulator receptor homologue AvaR3 plays a regulatory role in antibiotic production, mycelial aggregation and colony development of Streptomyces avermitilis. Microbiology. Vol.157, No.8 (2011), 2266-2275. doi:10.1099/mic.0.048371-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/12011
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
The autoregulator receptor homologue AvaR3 plays a regulatory role in antibiotic production, mycelial aggregation and colony development of Streptomyces avermitilis
Other Contributor(s)
Abstract
The γ-butyrolactone autoregulator receptor has been shown to control secondary metabolism and/or morphological differentiation across many Streptomyces species. Streptomyces avermitilis produces an important anthelmintic agent (avermectin) and two further polyketide antibiotics, filipin and oligomycin. Genomic analysis of S. avermitilis revealed that this micro-organism has the clustered putative autoregulator receptor genes distant from the antibiotic biosynthetic gene clusters. Here, we describe the characterization of avaR3, one of the clustered receptor genes, which encodes a protein containing an extra stretch of amino acid residues that has not been found in the family of autoregulator receptors. Disruption of avaR3 resulted in markedly decreased production of avermectins, with delayed expression of avermectin biosynthetic genes, suggesting that AvaR3 positively controls the avermectin biosynthetic genes. Moreover, the disruption caused increased production of filipin without any changes in the transcriptional profile of the filipin biosynthetic genes, suggesting that filipin production is indirectly controlled by AvaR3. The avaR3 disruptant displayed fragmented growth in liquid culture and conditional morphological defects on solid medium. These findings demonstrated that AvaR3 acts as a global regulator that controls antibiotic production and cell morphology. © 2011 SGM.