Publication: Production and characterization of genus and species specific monoclonal antibodies against surface tegumental antigens of Schistosoma mekongi
Issued Date
1994-01-01
Resource Type
ISSN
13653024
01419838
01419838
Other identifier(s)
2-s2.0-0028362483
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Parasite Immunology. Vol.16, No.4 (1994), 221-223
Suggested Citation
V. VIYANANT, P. SOBHON, E. S. UPATHAM Production and characterization of genus and species specific monoclonal antibodies against surface tegumental antigens of Schistosoma mekongi. Parasite Immunology. Vol.16, No.4 (1994), 221-223. doi:10.1111/j.1365-3024.1994.tb00343.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/9571
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Title
Production and characterization of genus and species specific monoclonal antibodies against surface tegumental antigens of Schistosoma mekongi
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Abstract
A panel of monoclonal antibodies (MoAbs) against freeze thaw surface tegumental antigens of Schistosoma mekongi were produced from naturally infected BALB/c mice. In this study, we have characterized two MoAbs which have different antigenic specificity for S. mekongi, S. japonicum and S. mansoni. The target epitopes of these two hybridoma antibodies are contained in the Mr 38 kDa (designated Sme 38) and Mr 97 kDa (designated Sme 97) proteins of adult worms as analysed by immunoblotting. The Sme 38 epitope was genus‐specific, since it is also detectable in S. japonicum and S. mansoni. The Sme 97 was not detected in S. japonicum and S. mansoni. therefore it is considered as species‐specific epitope. Immunocytochemical analysis revealed the presence of Sme 38 epitope in the surface tegument, the tegumental cells lying underneath the muscle layer and gut surface. The Sme 97 epitope was detectable only in the surface tegumental area. Copyright © 1994, Wiley Blackwell. All rights reserved