Publication: Short report: Monoclonal antibody-based immunofluorescence microscopy for the rapid identification of burkholderia pseudomallei in clinical specimens
Issued Date
2013-07-01
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ISSN
00029637
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2-s2.0-84880620964
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Mahidol University
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SCOPUS
Bibliographic Citation
American Journal of Tropical Medicine and Hygiene. Vol.89, No.1 (2013), 165-168
Suggested Citation
Sarunporn Tandhavanant, Gumphol Wongsuvan, Vanaporn Wuthiekanun, Nittaya Teerawattanasook, Nicholas P J Day, Direk Limmathurotsakul, Sharon J. Peacock, Narisara Chantratita Short report: Monoclonal antibody-based immunofluorescence microscopy for the rapid identification of burkholderia pseudomallei in clinical specimens. American Journal of Tropical Medicine and Hygiene. Vol.89, No.1 (2013), 165-168. doi:10.4269/ajtmh.13-0066 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31903
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Title
Short report: Monoclonal antibody-based immunofluorescence microscopy for the rapid identification of burkholderia pseudomallei in clinical specimens
Abstract
The diagnosis of melioidosis depends on the culture of Burkholderia pseudomallei, which takes at least 48 hours. We used a polyclonal-FITC-based immunofluorescence microscopic assay (Pab-IFA) on clinical samples to provide a rapid presumptive diagnosis. This has limitations including photobleaching and batch-to-batch variability. This study evaluated an IFA based on a monoclonal antibody specific to B. pseudomallei (Mab-IFA) and Alexa Fluor 488. A diagnostic evaluation was performed on a prospective cohort of 951 consecutive patients with suspected melioidosis. A total of 1,407 samples were tested. Test accuracy was defined against culture as the gold standard, and was also compared against Pab-IFA. A total of 88 samples from 64 patients were culture positive for B. pseudomallei. The diagnostic sensitivity and specificity of the Mab-IFA was comparable to the Pab-IFA (48.4% versus 45.3% for sensitivity, and 99.8% versus 98.8% for specificity). We have incorporated the Mab-IFA into our routine practice.© 2013 by The American Society of Tropical.