Publication: Interaction of the CDK2-associated protein-1, p12<sup>DOC-1/CDK2AP1</sup>, with its homolog, p14<sup>DOC-1R</sup>
Issued Date
2004-03-19
Resource Type
ISSN
0006291X
Other identifier(s)
2-s2.0-1342302367
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.315, No.4 (2004), 998-1003
Suggested Citation
Waranun Buajeeb, Xue Zhang, Hiroe Ohyama, David Han, Rudee Surarit, Yong Kim, David T.W. Wong Interaction of the CDK2-associated protein-1, p12<sup>DOC-1/CDK2AP1</sup>, with its homolog, p14<sup>DOC-1R</sup>. Biochemical and Biophysical Research Communications. Vol.315, No.4 (2004), 998-1003. doi:10.1016/j.bbrc.2004.02.003 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21214
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Title
Interaction of the CDK2-associated protein-1, p12<sup>DOC-1/CDK2AP1</sup>, with its homolog, p14<sup>DOC-1R</sup>
Abstract
Human DOC-1/CDK2AP1 gene encodes a growth suppressor protein of 12kDa (p12DOC-1/CDK2AP1). Recently, p12DOC-1/CDK2AP1has been shown to associate with cell cycle proteins including CDK2 and DNA polymerase α/primase. It negatively regulates CDK2 activities and suppresses DNA replication. Therefore, identification of other p12DOC-1/CDK2AP1interacting proteins might clarify its role in the cell cycle regulation and carcinogenesis. The purpose of this study was to identify additional p12DOC-1/CDK2AP1interacting proteins using the yeast two-hybrid system. Using human p12DOC-1/CDK2AP1as a bait in a liver cDNA library screening, cDNA clones identical to human DOC-1R transcript were identified. The interaction between p12DOC-1/CDK2AP1and p14DOC-1Rwas verified in vitro and in cells. GST pull-down assay and immunoprecipitation experiments confirmed the interaction between the two proteins. The critical region for p12DOC-1/CDK2AP1interaction with p14DOC-1Rwas defined to amino acids 20-25 by using a series of deletion mutants as baits in the yeast two-hybrid system. Our data indicated that p12DOC-1/CDK2AP1could associate with its homologous protein, p14DOC-1R. © 2004 Elsevier Inc. All rights reserved.