Publication: Decellularization enables characterization and functional analysis of extracellular matrix in planarian regeneration
Issued Date
2021-08-01
Resource Type
ISSN
15359484
15359476
15359476
Other identifier(s)
2-s2.0-85117948677
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular and Cellular Proteomics. Vol.20, (2021)
Suggested Citation
Ekasit Sonpho, Frederick G. Mann, Michaella Levy, Eric J. Ross, Carlos Guerrero-Hernández, Laurence Florens, Anita Saraf, Viraj Doddihal, Puey Ounjai, Alejandro Sánchez Alvarado Decellularization enables characterization and functional analysis of extracellular matrix in planarian regeneration. Molecular and Cellular Proteomics. Vol.20, (2021). doi:10.1016/J.MCPRO.2021.100137 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/76073
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Title
Decellularization enables characterization and functional analysis of extracellular matrix in planarian regeneration
Abstract
The extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions. However, only a few research organisms are available for the systematic dissection of the composition and functions of the ECM, particularly during regeneration. We utilized the free-living flatworm Schmidtea mediterranea to develop an integrative approach consisting of decellularization, proteomics, and RNAi to characterize and investigate ECM functions during tissue homeostasis and regeneration. ECM-enriched samples were isolated from planarians, and their proteomes were characterized by LC–MS/MS. The functions of identified ECM components were interrogated using RNA interference. Using this approach, we found that heparan sulfate proteoglycan is essential for tissue regeneration. Our strategy provides an experimental approach for identifying both known and novel ECM components involved in regeneration.