Publication: International laboratory comparison of influenza microneutralization assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) influenza viruses by CONSISE
Issued Date
2015-01-01
Resource Type
ISSN
1556679X
15566811
15566811
Other identifier(s)
2-s2.0-84939480223
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinical and Vaccine Immunology. Vol.22, No.8 (2015), 957-964
Suggested Citation
Karen L. Laurie, Othmar G. Engelhardt, John Wood, Alan Heath, Jacqueline M. Katz, Malik Peiris, Katja Hoschler, Olav Hungnes, Wenqing Zhang, Maria D. Van Kerkhove, Jaccqueline Katz, Xiuhua Lu, Min Levine, Vic Veguilla, Feng Liu, Yaohui Bai, Pilaipan Puthavathana, Hatairat Lerdsamran, Phisanu Pooruk, Knnika Nateerom, Maria Rita Castrucci, Isabella Donatelli, Marzia Facchini, Noriko Kishida, Masato Tashiro, Takato Odagiri, Shailesh D. Pawar, Sadhana S. Kode, Anthony Hawksworth, Ryan Ortiguerra, Gary Brice, Nicholas Martin, Tad Kochel, Jose Sanchez, Michael Cooper, James Cummings, Ralf Wagner, Constanze Goepfert, Nina Alex, Joanna Hammann, Britta Neumann, Mahendra Perera, Emanuele Montomoli, Guilia Lapini, Sara Sbragi, Tian Bai, Zaijiang Yu, Jianfang Zhou, Louise Carolan, Karen Laurie International laboratory comparison of influenza microneutralization assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) influenza viruses by CONSISE. Clinical and Vaccine Immunology. Vol.22, No.8 (2015), 957-964. doi:10.1128/CVI.00278-15 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35654
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Title
International laboratory comparison of influenza microneutralization assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) influenza viruses by CONSISE
Author(s)
Karen L. Laurie
Othmar G. Engelhardt
John Wood
Alan Heath
Jacqueline M. Katz
Malik Peiris
Katja Hoschler
Olav Hungnes
Wenqing Zhang
Maria D. Van Kerkhove
Jaccqueline Katz
Xiuhua Lu
Min Levine
Vic Veguilla
Feng Liu
Yaohui Bai
Pilaipan Puthavathana
Hatairat Lerdsamran
Phisanu Pooruk
Knnika Nateerom
Maria Rita Castrucci
Isabella Donatelli
Marzia Facchini
Noriko Kishida
Masato Tashiro
Takato Odagiri
Shailesh D. Pawar
Sadhana S. Kode
Anthony Hawksworth
Ryan Ortiguerra
Gary Brice
Nicholas Martin
Tad Kochel
Jose Sanchez
Michael Cooper
James Cummings
Ralf Wagner
Constanze Goepfert
Nina Alex
Joanna Hammann
Britta Neumann
Mahendra Perera
Emanuele Montomoli
Guilia Lapini
Sara Sbragi
Tian Bai
Zaijiang Yu
Jianfang Zhou
Louise Carolan
Karen Laurie
Othmar G. Engelhardt
John Wood
Alan Heath
Jacqueline M. Katz
Malik Peiris
Katja Hoschler
Olav Hungnes
Wenqing Zhang
Maria D. Van Kerkhove
Jaccqueline Katz
Xiuhua Lu
Min Levine
Vic Veguilla
Feng Liu
Yaohui Bai
Pilaipan Puthavathana
Hatairat Lerdsamran
Phisanu Pooruk
Knnika Nateerom
Maria Rita Castrucci
Isabella Donatelli
Marzia Facchini
Noriko Kishida
Masato Tashiro
Takato Odagiri
Shailesh D. Pawar
Sadhana S. Kode
Anthony Hawksworth
Ryan Ortiguerra
Gary Brice
Nicholas Martin
Tad Kochel
Jose Sanchez
Michael Cooper
James Cummings
Ralf Wagner
Constanze Goepfert
Nina Alex
Joanna Hammann
Britta Neumann
Mahendra Perera
Emanuele Montomoli
Guilia Lapini
Sara Sbragi
Tian Bai
Zaijiang Yu
Jianfang Zhou
Louise Carolan
Karen Laurie
Other Contributor(s)
Victorian Infectious Diseases Reference Laboratory
National Institute for Biological Standards and Control
Centers for Disease Control and Prevention
The University of Hong Kong
Public Health England
Norwegian Institute of Public Health
Organisation Mondiale de la Sante
Institut Pasteur, Paris
Mahidol University
Istituto Superiore Di Sanita
National Institute of Infectious Diseases
National Institute of Virology India
U.S. Naval Health Research Center
Naval Medical Research Center
Paul-Ehrlich-Institut
Universita degli Studi di Siena
Chinese National Influenza Center
National Institute for Biological Standards and Control
Centers for Disease Control and Prevention
The University of Hong Kong
Public Health England
Norwegian Institute of Public Health
Organisation Mondiale de la Sante
Institut Pasteur, Paris
Mahidol University
Istituto Superiore Di Sanita
National Institute of Infectious Diseases
National Institute of Virology India
U.S. Naval Health Research Center
Naval Medical Research Center
Paul-Ehrlich-Institut
Universita degli Studi di Siena
Chinese National Influenza Center
Abstract
Copyright © 2015, American Society for Microbiology. All Rights Reserved. The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HAMNassay protocols to enable better correlation of these assays in the future.