Publication: Plasmodium berghei: Uptake and distribution of chloroquine in infected mouse erythrocytes
Issued Date
1982-01-01
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ISSN
10902449
00144894
00144894
Other identifier(s)
2-s2.0-0020434348
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Mahidol University
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SCOPUS
Bibliographic Citation
Experimental Parasitology. Vol.54, No.2 (1982), 260-270
Suggested Citation
W. Sirawaraporn, B. Panijpan, Y. Yuthavong Plasmodium berghei: Uptake and distribution of chloroquine in infected mouse erythrocytes. Experimental Parasitology. Vol.54, No.2 (1982), 260-270. doi:10.1016/0014-4894(82)90135-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30329
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Title
Plasmodium berghei: Uptake and distribution of chloroquine in infected mouse erythrocytes
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Abstract
The capacity of mouse erythrocytes infected with Plasmodium berghei to accumulate chloroquine is developed with maturation of the parasites. This is shown by direct comparison of the early and mature stages, which are separated by density difference. After drug accumulation, infected cells were fractionated by saponin lysis or nitrogen decompression to study the drug distribution. Effectiveness of isolating intact parasites and host components was checked by SDS-polyacrylamide gel electrophoresis and by low leakage of parasite-specific lactate dehydrogenase used as a marker enzyme. At low external drug concentration (~10-7M), chloroquine is principally accumulated in the parasites. However, at higher drug concentrations (~10-5and ~10-3M), the proportion of the drug found in the host cytosol fraction is increased. A small but significant proportion of the drug (<20%) is associated with the host cell membrane. The pellet fraction of the freed parasites, further fractionated by freeze-thaw lysis, contains a major proportion of the drug at low external concentrations. However, the pellet fraction obtained from prolonged sonication of the parasites, which contains the bulk of hemozoin pigment, carries only a small proportion of the drug. This indicates that parasite membrane components may bind most of the drug. As external chloroquine concentration is increased, the proportion of drug in the parasite supernatant increases, some or most of which is probably bound by soluble hemecontaining compounds. However, the presence of chloroquine in the parasite does not affect the partition of heme in particulate and soluble forms. © 1982.