Publication: Evaluation of an in-house immunoperoxidase staining assay for histodiagnosis of human pythiosis
1
Issued Date
2009-11-01
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-76749145087
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.6 (2009), 1298-1305
Suggested Citation
Angsana Keeratijarut, Patcharee Karnsombut, Rangsima Aroonroch, Somboon Srimuang, Tumtip Sangruchi, Lalana Sansopha, Piroon Mootsikapun, Noppadol Larbcharoensub, Theerapong Krajaejun Evaluation of an in-house immunoperoxidase staining assay for histodiagnosis of human pythiosis. Southeast Asian Journal of Tropical Medicine and Public Health. Vol.40, No.6 (2009), 1298-1305. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/27884
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Evaluation of an in-house immunoperoxidase staining assay for histodiagnosis of human pythiosis
Other Contributor(s)
Abstract
Pythiosis, a life-threatening infectious disease of humans and animals in tropical and subtropical countries, is caused by the fungus-like organism Pythium insidiosum. As diagnosis of pythiosis is difficult, delayed diagnosis of pythiosis leads to poor prognosis. We developed an immunoperoxidase staining assay using rabbit anti-P. insidiosum antibodies to detect P. insidiosum directly in infected tissues of 19 patients with vascular (n = 11), ocular (n = 7) or cutaneous (n = 1) pythiosis. Tissue sections from 31 patients with various fungal infections were included as controls. Tissue sections from all pythiosis patients and 2 patients with Fusarium infections were stained positive, whereas the other 29 control sections were stained negative. Sensitivity and specificity of the assay was 100% and 94%, respectively. Based on the prevalence of human pythiosis (2%), calculated positive predictive value and negative predictive value was 24% and 100%, respectively. Thus, the diagnostic value of this assay is for ruling out pythiosis. The assay requires routine laboratory equipments and can easily be performed by pathologists in rural hospitals where the disease is more prevalent.