Publication:
Anti-MSP11 IgG inhibits Plasmodium falciparum merozoite invasion into erythrocytes in vitro

dc.contributor.authorTatsuhiro Tohmotoen_US
dc.contributor.authorEizo Takashimaen_US
dc.contributor.authorSatoru Takeoen_US
dc.contributor.authorMasayuki Moritaen_US
dc.contributor.authorHikaru Nagaokaen_US
dc.contributor.authorRachanee Udomsangpetchen_US
dc.contributor.authorJetsumon Sattabongkoten_US
dc.contributor.authorTomoko Ishinoen_US
dc.contributor.authorMotomi Toriien_US
dc.contributor.authorTakafumi Tsuboien_US
dc.contributor.otherKyorin Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherEhime Universityen_US
dc.date.accessioned2020-01-27T08:59:03Z
dc.date.available2020-01-27T08:59:03Z
dc.date.issued2019-04-01en_US
dc.description.abstract© 2018 Elsevier B.V. Merozoite surface proteins (MSPs) are considered as promising blood-stage malaria vaccine candidates. MSP3 has long been evaluated for its vaccine candidacy, however, the candidacy of other members of MSP3 family is insufficiently characterized. Here, we investigated Plasmodium falciparum MSP11 (PF3D7_1036000), a member of the MSP3 family, for its potential as a blood-stage vaccine candidate. The full-length protein (MSP11-FL) as well as the N-terminal half-MSP11 (MSP11-N), known to be unique among the MSP3 family members, were expressed by wheat germ cell-free system, and used to raise antibodies in rabbit. Immunoblot analysis of schizont lysates probed with anti-MSP11-N antibodies detected double bands at approximately 40 and 60 kDa, consistent with the previous report thus confirming antibodies specificity. However, inconsistent with previously reported merozoite's surface localization, immunofluorescence assay (IFA) revealed that MSP11 likely localizes to rhoptry neck of merozoites in mature schizonts. After invasion, MSP11 localized to parasitophorous vacuole and thereafter in Maurer's clefts in trophozoites. Anti-MSP11-FL antibody levels were significantly higher in asymptomatic than symptomatic P. falciparum cases in malaria low endemic Thailand. This reconfirmed that anti-MSP11 antibodies play an important role in protection against clinical malaria, as previously reported. Furthermore, in vitro growth inhibition assay revealed that anti-MSP11-FL rabbit antibodies biologically function by inhibiting merozoite invasion of erythrocytes. These findings further support the vaccine candidacy of MSP11.en_US
dc.identifier.citationParasitology International. Vol.69, (2019), 25-29en_US
dc.identifier.doi10.1016/j.parint.2018.10.012en_US
dc.identifier.issn18730329en_US
dc.identifier.issn13835769en_US
dc.identifier.other2-s2.0-85056856503en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/51083
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85056856503&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleAnti-MSP11 IgG inhibits Plasmodium falciparum merozoite invasion into erythrocytes in vitroen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85056856503&origin=inwarden_US

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