Publication: Pueraria mirifica leaves, an alternative potential isoflavonoid source
Issued Date
2014-01-01
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ISSN
13476947
09168451
09168451
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2-s2.0-84925659426
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioscience, Biotechnology and Biochemistry. Vol.78, No.6 (2014), 917-926
Suggested Citation
Jutarmas Jungsukcharoen, Binar Asrining Dhiani, Wichai Cherdshewasart, Nawaporn Vinayavekhin, Polkit Sangvanich, Chuenchit Boonchird Pueraria mirifica leaves, an alternative potential isoflavonoid source. Bioscience, Biotechnology and Biochemistry. Vol.78, No.6 (2014), 917-926. doi:10.1080/09168451.2014.910091 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33389
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Title
Pueraria mirifica leaves, an alternative potential isoflavonoid source
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Abstract
© 2014 Japan Society for Bioscience, Biotechnology, and Agrochemistry. We investigated the major leaf isoflavonoid contents of Pueraria mirifica from three different cultivars (PM-III, PM-IV, and PM-V) using reverse RP-HPLC analysis. The proportions and net levels of puerarin, daidzin, genistin, and daidzein in P. mirifica leaves were found to depend on the plant cultivar and to correlate with cultivation temperature and rainfall amount. The crude leaf-extracts were tested using the Yeast Estrogen Screen (YES) assay with both human estrogen receptors (hERα and hERp). Their estrogenic activity was higher when determined by the YES system containing hERp than that with hERα and was also higher when the Asnq2 than the wildtype yeast was employed. The results open the possibility of selecting and cultivating certain P. mirifica cultivars at a farm scale to produce a sufficient supply of leaf material to act as a starting source for the commercial scale extraction of these major isoflavonoids.