Publication: Factorial design applied to a non-aqueous capillary electrophoresis method for the separation of β-agonists
Issued Date
2006-11-17
Resource Type
ISSN
00219673
Other identifier(s)
2-s2.0-33750990469
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Chromatography A. Vol.1134, No.1-2 (2006), 326-332
Suggested Citation
Oraphan Anurukvorakun, Worapot Suntornsuk, Leena Suntornsuk Factorial design applied to a non-aqueous capillary electrophoresis method for the separation of β-agonists. Journal of Chromatography A. Vol.1134, No.1-2 (2006), 326-332. doi:10.1016/j.chroma.2006.09.021 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23140
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Factorial design applied to a non-aqueous capillary electrophoresis method for the separation of β-agonists
Other Contributor(s)
Abstract
The aim of this work was to study the effects of both chemical and instrumental parameters on the separation of β-agonists (clenbuterol (CLE), salbutamol (SAL) and terbutaline (TER)) by non-aqueous capillary electrophoresis (NACE) method. Due to the number of parameters involved and their interactions, factorial experimental designs (EDs) at two levels was applied to investigate the influence of experimental factors (ionic strength of the background electrolyte (BGE), organic solvent, injection time, voltage and temperature) in sets of several CE responses (resolution, (RS), number of theoretical plate (N), tailing factor (TF) and migration time (tm)). As a compromise between the four responses, the optimum condition was obtained in 18 mM ammonium acetate in methanol (MeOH):acetonitrile (ACN):glacial acetic acid (66:33:1%, v/v/v) using an injection time of 4 s, the voltage and the temperature of 28 kV and 24 °C, respectively. The proposed NACE permitted the baseline separation of the three β-agonists within 10.5 min with good repeatability (%RSD < 3.5%) and linearity (r2> 0.99). The developed method was applicable for the analysis of the β-agonists in syrup and tablets and the NACE condition was compatible with a mass spectrometer detector. © 2006 Elsevier B.V. All rights reserved.