Publication:
Stage-dependent production and release of histidine-rich protein 2 by Plasmodium falciparum

dc.contributor.authorVarunee Desakornen_US
dc.contributor.authorArjen M. Dondorpen_US
dc.contributor.authorKamolrat Silamuten_US
dc.contributor.authorWirichada Pongtavornpinyoen_US
dc.contributor.authorDuangjai Sahassanandaen_US
dc.contributor.authorKesinee Chotivanichen_US
dc.contributor.authorPunnee Pitisuttithumen_US
dc.contributor.authorA. M. Smithymanen_US
dc.contributor.authorNicholas P J Dayen_US
dc.contributor.authorNicholas J. Whiteen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherNuffield Department of Clinical Medicineen_US
dc.contributor.otherCellabs Pty Ltd.en_US
dc.date.accessioned2018-06-21T08:15:48Z
dc.date.available2018-06-21T08:15:48Z
dc.date.issued2005-07-01en_US
dc.description.abstractBecause of their sequestration in the microcirculation, the pathogenic late stages of Plasmodium falciparum are under-represented in peripheral blood samples from patients with falciparum malaria. Excreted products of the parasite might help to estimate this sequestered biomass. We quantified the stage-dependent production and release per parasite of P. falciparum histidine-rich protein 2 (PfHRP2) with the objective of measuring the sequestered biomass. A simple method to relate parasite stage to parasite age was developed to facilitate this. In four isolates of P. falciparum, the median (range) PfHRP2 content was 2.0 fg (0.5-4.3 fg) for a young ring stage infected erythrocyte, and 5.4 fg (2.1-10.2 fg) for the schizont stage. The amount of PfHRP2 in the parasitized erythrocyte increased most during development to the mature trophozoite stage. The median (range) amount of PfHRP2 secreted per parasite per entire erythrocytic cycle was 5.2 fg (1.1-13.0 fg). A median of 89% of the total PfHRP2 was excreted at the moment of schizont rupture. This assessment of the stage-dependent release of PfHRP2 is an essential prerequisite for future studies aimed at estimating the total patient parasite mass from the peripheral blood PfHRP2 concentration. © 2005 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.en_US
dc.identifier.citationTransactions of the Royal Society of Tropical Medicine and Hygiene. Vol.99, No.7 (2005), 517-524en_US
dc.identifier.doi10.1016/j.trstmh.2004.11.014en_US
dc.identifier.issn00359203en_US
dc.identifier.other2-s2.0-21044447731en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/16573
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=21044447731&origin=inwarden_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.titleStage-dependent production and release of histidine-rich protein 2 by Plasmodium falciparumen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=21044447731&origin=inwarden_US

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