Publication: Role of altholactone in inducing type II apoptosis signalling pathway and expression of cancer-related genes in cervical carcinoma HeLa cell line
Issued Date
2013-05-29
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ISSN
10958355
10656995
10656995
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2-s2.0-84878137096
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Mahidol University
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SCOPUS
Bibliographic Citation
Cell Biology International. Vol.37, No.5 (2013), 471-477
Suggested Citation
Wanlaya Uthaisang-Tanechpongtamb, Promjit Sriyabhaya, Prapon Wilairat Role of altholactone in inducing type II apoptosis signalling pathway and expression of cancer-related genes in cervical carcinoma HeLa cell line. Cell Biology International. Vol.37, No.5 (2013), 471-477. doi:10.1002/cbin.10059 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31308
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Title
Role of altholactone in inducing type II apoptosis signalling pathway and expression of cancer-related genes in cervical carcinoma HeLa cell line
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Abstract
Goniothalamus species (Annonaceae) is a shrub that grows in the rainforest of tropical Asia. Several compounds have been isolated and exhibit the potential use for cancer treatment. In this work, altholactone isolated from Goniothalamus macrophyllus was investigated for its cytotoxicity, apoptosis signalling and the expression of cancer-related genes in the cervical carcinoma HeLa cells. Cytotoxicity was evaluated by MTT assay. Apoptotic characteristics were evaluated by morphological studies. Caspase-3 activity was detected using a fluorogenic substrate. Cytochrome c release from mitochondria and protein Bid were determined byWestern blotting and cancer-related genes expression by RT-PCR. The results demonstrated that altholactone was cytotoxic to HeLa (IC50 = 9.6 μmg/mL), and apoptotic cell death was manifested by appearance of chromatin condensation and caspase-3 activation, which was inhibited by specific inhibitors of both caspase-8 and -9. Release into the cytosol of cytochrome and cleavage of Bid occurred. Altholactone also caused a decrease in bcl-2 and an increase in p53 expression. These unique properties of altholactone suggest a potential for cancer chemotherapy. © 2013 International Federation for Cell Biology.