Publication: Enzymatic responses of the riceland prawn, Macrobrachium lanchesteri, to chlorpyrifos exposure
Issued Date
2012-08-01
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ISSN
13369563
00063088
00063088
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2-s2.0-84863326428
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Mahidol University
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SCOPUS
Bibliographic Citation
Biologia. Vol.67, No.4 (2012), 762-766
Suggested Citation
Wuttipong Tongbai, Rapee Boonplueng, Praneet Damrongphol Enzymatic responses of the riceland prawn, Macrobrachium lanchesteri, to chlorpyrifos exposure. Biologia. Vol.67, No.4 (2012), 762-766. doi:10.2478/s11756-012-0061-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13418
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Title
Enzymatic responses of the riceland prawn, Macrobrachium lanchesteri, to chlorpyrifos exposure
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Abstract
Cholinesterase (ChE) was characterized in whole bodies of adult riceland prawns, Macrobrachium lanchesteri, based on substrate preference and inhibitor sensitivity. M. lanchesteri ChE activity was mainly attributable to acetylcholinesterase (AChE) since it preferentially hydrolyzed an AChE-specific substrate, acetylthiocholine iodide, over s-butyrylthiocholine iodide and was sensitive to 1,5-bis-(4-allyldimethyl-ammoniumphenyl)-pentan-3-one dibromide, a specific inhibitor for AChE. The effect of chlorpyrifos exposure on M. lanchesteri were also investigated. The 24, 48, 72 and 96 h LC 50 values for chlorpyrifos were 3. 37, 2. 76, 2. 58 and 2. 53 μg L -1 , respectively. Based on these values, adult M. lanchesteri were exposed to 0. 5, 1. 5, 2. 5 and 3. 5 μg chlorpyrifos L -1 for 24, 48, 72 and 96 h. Chlorpyrifos appeared to induce drastic enzymatic responses in M. lanchesteri. AChE activity was inhibited after 24 h of exposure in a dose- and time-dependent manner. The levels of lipid peroxidation increased significantly after 24 h of exposure. However, the activities of the antioxidant enzyme, catalase, and the detoxification enzyme, glutathione S-transferase, were reduced. In conclusion, M. lanchesteri is sensitive to chlorpyrifos and can serve as a bioindicator species for freshwater environmental assessment. © 2012 Versita Warsaw and Springer-Verlag Wien.