Publication: Rapid determination of viral RNA sequences in mosquitoes collected in the field
Issued Date
2007-12-01
Resource Type
ISSN
01660934
Other identifier(s)
2-s2.0-35649010953
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.146, No.1-2 (2007), 372-374
Suggested Citation
Yuki Kihara, Tomomitsu Satho, Yuki Eshita, Kouji Sakai, Akira Kotaki, Tomohiko Takasaki, Yupha Rongsriyam, Narumon Komalamisra, Raweewan Srisawat, Parichat Lapcharoen, Suchada Sumroiphon, Shiroh Iwanaga, Hiroshi Ushijima, Daiji Endoh, Takeshi Miyata, Akira Sakata, Nobuhiro Kashige, Fumio Miake, Shuetsu Fukushi, Masayuki Saijo, Ichiro Kurane, Shigeru Morikawa, Tetsuya Mizutani Rapid determination of viral RNA sequences in mosquitoes collected in the field. Journal of Virological Methods. Vol.146, No.1-2 (2007), 372-374. doi:10.1016/j.jviromet.2007.07.008 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24479
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Title
Rapid determination of viral RNA sequences in mosquitoes collected in the field
Author(s)
Yuki Kihara
Tomomitsu Satho
Yuki Eshita
Kouji Sakai
Akira Kotaki
Tomohiko Takasaki
Yupha Rongsriyam
Narumon Komalamisra
Raweewan Srisawat
Parichat Lapcharoen
Suchada Sumroiphon
Shiroh Iwanaga
Hiroshi Ushijima
Daiji Endoh
Takeshi Miyata
Akira Sakata
Nobuhiro Kashige
Fumio Miake
Shuetsu Fukushi
Masayuki Saijo
Ichiro Kurane
Shigeru Morikawa
Tetsuya Mizutani
Tomomitsu Satho
Yuki Eshita
Kouji Sakai
Akira Kotaki
Tomohiko Takasaki
Yupha Rongsriyam
Narumon Komalamisra
Raweewan Srisawat
Parichat Lapcharoen
Suchada Sumroiphon
Shiroh Iwanaga
Hiroshi Ushijima
Daiji Endoh
Takeshi Miyata
Akira Sakata
Nobuhiro Kashige
Fumio Miake
Shuetsu Fukushi
Masayuki Saijo
Ichiro Kurane
Shigeru Morikawa
Tetsuya Mizutani
Abstract
A method for rapid determination of viral RNA sequences (RDV) was applied to homogenates of Aedes aegypti collected in Thailand in an area in which dengue fever (dengue hemorrhagic fever) is endemic, using the mosquito cell line C6/36. Nucleic acid sequences of dengue virus type 4 and cell fusing agent virus were detected. This RDV method has the potential to become a standard method for detection of both known and newly emerging, unknown mosquito-borne viruses. © 2007.