Effects of an oil spill on the leaf anatomical characteristics of a beach plant (Terminalia catappa L.)

Abstract

© 2017, Springer-Verlag GmbH Germany. This study investigated the short-term impacts of an oil spill on the leaf anatomical structures of Terminalia catappa L. from crude oil leakage in Rayong province, Thailand, in 2013. Approximately 3 weeks after the oil spill, leaves of T. catappa were collected along the coastline of Rayong from one affected site, five adjacent sites, and a control site. Slides of the leaf epidermis were prepared by the peeling method, while leaf and petiole transverse sections were prepared by paraffin embedding. Cell walls of adaxial epidermal cell on leaves in the affected site were straight instead of the jigsaw shape found in leaves from the adjacent and control sites. In addition, the stomatal index of the abaxial leaf surface was significantly lower in the affected site. Leaf and petiole transverse sections collected from the affected site showed increased cuticle thickness, epidermal cell diameter on both sides, and palisade mesophyll thickness; in contrast, vessel diameter and spongy mesophyll thickness were reduced. These significant changes in the leaf anatomy of T. catappa correspond with previous research and demonstrate the negative effects of oil spill pollution on plants. The anatomical changes of T. catappa in response to crude oil pollution are discussed as a possible indicator of pollution and may be used in monitoring crude oil pollution.