Publication: Testosterone protects against glucotoxicity-induced apoptosis of pancreatic β-cells (INS-1) and male mouse pancreatic islets
Issued Date
2013-11-01
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ISSN
19457170
00137227
00137227
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2-s2.0-84886561445
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Mahidol University
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SCOPUS
Bibliographic Citation
Endocrinology. Vol.154, No.11 (2013), 4058-4067
Suggested Citation
Wanthanee Hanchang, Namoiy Semprasert, Thawornchai Limjindaporn, Pa Thai Yenchitsomanus, Suwattanee Kooptiwut Testosterone protects against glucotoxicity-induced apoptosis of pancreatic β-cells (INS-1) and male mouse pancreatic islets. Endocrinology. Vol.154, No.11 (2013), 4058-4067. doi:10.1210/en.2013-1351 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31171
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Title
Testosterone protects against glucotoxicity-induced apoptosis of pancreatic β-cells (INS-1) and male mouse pancreatic islets
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Abstract
Male hypogonadism associates with type 2 diabetes, and T can protect pancreatic β-cells from glucotoxicity. However, the protective mechanism is still unclear. This study thus aims to examine the antiapoptotic mechanism of T in pancreatic β cells cultured in high-glucose medium. T (0.0005-2 μg/mL) was added to INS-1 cells cultured in basal glucose or high-glucose media. Then cellular apoptosis, oxidative stress, and cell viability were measured. Endoplasmic reticulum (ER) stress markers and sensors and the antiapoptotic protein (B-cell lymphoma 2) were investigated by real-time PCR and Western blot analysis. ER stress markers were also measured in male mouse pancreatic islet cultured in similar conditions. T (0.05 and 0.5 μg/mL) did not have any effect on apoptosisandviability of INS-1 cells cultured in basal glucose medium, but it could reduce apoptosis and increase viability of INS-1 cells cultured in high-glucose medium. The protective effect of T is diminished by androgen receptor inhibitor. T (0.05μg/mL) could significantly reduce nitrotyrosine levels, mRNA, and protein levels of the ER stress markers and sensor those that were induced when INS-1 cells were cultured in high-glucose medium. It could also significantly increase the survival proteins, sarco/endoplasmic reticulum Ca2+ ATPase-2, and B-cell lymphoma 2 in INS-1 cells cultured in the same conditions. Similarly, it could reduce ER stress markers and increase sarco/endoplasmic reticulum Ca2+ ATPase protein levels in male mouse pancreatic islets cultured in high-glucose medium. T can protect against male pancreaticβ-cell apoptosis from glucotoxicity via the reduction of both oxidative stress and ER stress. Copyright © 2013 by The Endocrine Society.