Publication: Simultaneous overexpression of multidrug efflux pumps in Pseudomonas aeruginosa non-cystic fibrosis clinical isolates
Issued Date
2014-01-01
Resource Type
ISSN
14803275
00084166
00084166
Other identifier(s)
2-s2.0-84904248550
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Canadian Journal of Microbiology. Vol.60, No.7 (2014), 437-443
Suggested Citation
Kanchana Poonsuk, Chanwit Tribuddharat, Rungtip Chuanchuen Simultaneous overexpression of multidrug efflux pumps in Pseudomonas aeruginosa non-cystic fibrosis clinical isolates. Canadian Journal of Microbiology. Vol.60, No.7 (2014), 437-443. doi:10.1139/cjm-2014-0239 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33371
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Simultaneous overexpression of multidrug efflux pumps in Pseudomonas aeruginosa non-cystic fibrosis clinical isolates
Other Contributor(s)
Abstract
The purpose of this study was to examine expression and regulation of 6 multidrug efflux systems, including MexAB-OprM, MexCD-OprJ, MexEF-OprN, MexXY, MexJK, and MexVW, in 13 non-cystic fibrosis (CF) clinical isolates of Pseudomonas aeruginosa. These isolates displayed a high level of resistance to many clinically important antibiotics. Some isolates simultaneously overexpressed up to 4 different Mex systems, as determined by quantitative real-time reverse transcription PCR. None of the isolates overexpressed MexCD-OprJ, and only 1 isolate overproduced MexJK. All the isolates overexpressed MexXY, while overexpression of MexEF-OprN and MexVW was common. DNA sequencing analysis of regulatory genes showed that no clear correlation could be established among (i) the presence of mutations, (ii) the type of mutations, (iii) the expression level of the Mex systems, and (iv) resistance to antibiotic substrates. The results suggest that the concomitant overexpression of some Mex systems may superimpose their antimicrobial drug efflux capabilities, contributing to the multidrug resistance phenotype in the P. aeruginosa non-CF clinical isolates. The existence of uncharacterized regulators for the Mex systems was signified.