Publication: Fluorescence detection of deoxyadenosine in Cordyceps spp. By indicator displacement assay
Issued Date
2020-05-01
Resource Type
ISSN
14203049
Other identifier(s)
2-s2.0-85084058808
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Molecules. Vol.25, No.9 (2020)
Suggested Citation
Arinta Agnie Dewantari, Nattha Yongwattana, Panwajee Payongsri, Sawinee Seemakhan, Suparerk Borwornpinyo, Akio Ojida, Jirarut Wongkongkatep Fluorescence detection of deoxyadenosine in Cordyceps spp. By indicator displacement assay. Molecules. Vol.25, No.9 (2020). doi:10.3390/molecules25092045 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/56115
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Fluorescence detection of deoxyadenosine in Cordyceps spp. By indicator displacement assay
Other Contributor(s)
Abstract
© 2020 by the authors. A rapid, sensitive and reliable indicator displacement assay (IDA) for specific detection of 2'- and 3'-deoxyadenosine (2'-dAde and 3'-dAde), the latter is also known as cordycepin, was established. The formation of inclusion complex between protonated acridine orange (AOH+) and cucurbit[7]uril (CB7) resulted in the hypochromic shift of fluorescent emission from 530 nm to 512 nm. Addition of cordycepin to the highly fluorescent AOH+/CB7 complex resulted in a unique tripartite AOH+/CB7/dAde complex with diminished fluorescence, and such reduction in emission intensity serves as the basis for our novel sensing system. The detection limits were 11 and 82 µM for 2'- and 3'-deoxyadenosine, respectively. The proposed method also demonstrated high selectivity toward 2'- and 3'-deoxyadenosine, owing to the inability of other deoxynucleosides, nucleosides and nucleotides commonly found in Cordyceps spp. to displace the AOH+ from the AOH+/CB7 complex, which was confirmed by isothermal titration calorimetry (ITC), UV-Visible and proton nuclear magnetic resonance (1H-NMR) spectroscopy. Our method was successfully implemented in the analysis of cordycepin in commercially available Ophiocordyceps and Cordyceps supplements, providing a novel and effective tool for quality assessment of these precious fungi with several health benefits.