Publication: Novel, anionic, antiviral septapeptides from mosquito cells also protect monkey cells against dengue virus
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Issued Date
2013-06-01
Resource Type
ISSN
18729096
01663542
01663542
Other identifier(s)
2-s2.0-84877273855
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Antiviral Research. Vol.98, No.3 (2013), 449-456
Suggested Citation
Chaowanee Laosutthipong, Nipaporn Kanthong, Timothy W. Flegel Novel, anionic, antiviral septapeptides from mosquito cells also protect monkey cells against dengue virus. Antiviral Research. Vol.98, No.3 (2013), 449-456. doi:10.1016/j.antiviral.2013.04.011 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31912
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Title
Novel, anionic, antiviral septapeptides from mosquito cells also protect monkey cells against dengue virus
Abstract
We have shown previously that ultrafiltrates (5. kDa cutoff) of cell-free medium from mosquito cell cultures persistently infected with DENV serotype 2 (DENV-2) contained a novel antiviral agent (called viprolaxikine) that could protect pre-treated, naïve mosquito cells from DENV infection. Here, we show that viprolaxikine also reduced DENV-2 titers by almost 4 logs (>99.9%) when compared to Vero cells mock-treated with ultrafiltrates from cultures of uninfected mosquito cells. Protease treatment removed the anti-DENV-2 activity. Pre-incubation for 48-h was required to obtain the maximum, dose-dependent protection against DENV-2, indicating that the antiviral activity was based on the interaction between Vero cells and viprolaxikine rather than direct action of viprolaxikine on DENV-2. Activity was highest against DENV-2, but there was also significant activity against the 3 other DENV serotypes. LC-MS-MS analysis revealed that the active viprolaxikine fraction contained anionic, antiviral peptides, each comprised of 7 amino acids (DDHELQD, DETELQD and DEVMLQD or DEVLMQD) and with a common sequence motif of D-D/E-X-X-X-Q-D. These sequences do not occur in the dengue virus genome, suggesting that the peptides are produced by the host insect cells when persistently infected with DENV-2. These peptides represent a new class of anionic, insect-derived, antiviral peptides with activity against a flavivirus in both mammalian and insect cells. © 2013 Elsevier B.V.