Publication: Development of a SYBR Green I-based real-time PCR for detection of elephant endotheliotropic herpesvirus 1 infection in Asian elephants (Elephas maximus)
2
Issued Date
2012-10-01
Resource Type
ISSN
18790984
01660934
01660934
Other identifier(s)
2-s2.0-84864312058
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.185, No.1 (2012), 160-165
Suggested Citation
Ladawan Sariya, Jarin Chatsirivech, Parut Suksai, Witthawat Wiriyarat, Adisak Songjaeng, Siriporn Tangsudjai, Oraphan Kanthasaewee, Umaporn Maikaew, Kridsada Chaichoun Development of a SYBR Green I-based real-time PCR for detection of elephant endotheliotropic herpesvirus 1 infection in Asian elephants (Elephas maximus). Journal of Virological Methods. Vol.185, No.1 (2012), 160-165. doi:10.1016/j.jviromet.2012.06.005 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/14256
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Title
Development of a SYBR Green I-based real-time PCR for detection of elephant endotheliotropic herpesvirus 1 infection in Asian elephants (Elephas maximus)
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Abstract
Elephant endotheliotropic herpesvirus 1 (EEHV1) can cause fatal hemorrhagic disease in Asian elephants (Elephas maximus). Several studies have described this virus as a major threat to young Asian elephants. A SYBR Green I-based real-time polymerase chain reaction (PCR) was developed to identify EEHV1 on trunk swabs and necropsied tissues. Two of 29 (6.9%) trunk swab samples from healthy Asian elephants were positive for EEHV1. The viruses were analyzed and classified as EEHV1A based on 231 nucleotides of the terminase gene. Necropsied spleen and heart tissue showed the highest level and second highest levels of DNA virus copy accumulation, respectively. The detection limit of the test was 276. copies/μl of DNA. There was no cross-reaction with other mammalian herpesviruses, such as herpes simplex virus 1 and equine herpesvirus 2. Inter- and intra-assay showed low coefficients of variation values indicating the reproducibility of the test. The results indicated that the test can be practically used for epidemiological study, clinical diagnosis, and management and control of EEHV1. © 2012 Elsevier B.V..