Publication: Rational Zika vaccine design via the modulation of antigen membrane anchors in chimpanzee adenoviral vectors
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Issued Date
2018-12-01
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ISSN
20411723
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2-s2.0-85048986838
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Mahidol University
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SCOPUS
Bibliographic Citation
Nature Communications. Vol.9, No.1 (2018)
Suggested Citation
César López-Camacho, Peter Abbink, Rafael A. Larocca, Wanwisa Dejnirattisai, Michael Boyd, Alex Badamchi-Zadeh, Zoë R. Wallace, Jennifer Doig, Ricardo Sanchez Velazquez, Roberto Dias Lins Neto, Danilo F. Coelho, Young Chan Kim, Claire L. Donald, Ania Owsianka, Giuditta De Lorenzo, Alain Kohl, Sarah C. Gilbert, Lucy Dorrell, Juthathip Mongkolsapaya, Arvind H. Patel, Gavin R. Screaton, Dan H. Barouch, Adrian V.S. Hill, Arturo Reyes-Sandoval Rational Zika vaccine design via the modulation of antigen membrane anchors in chimpanzee adenoviral vectors. Nature Communications. Vol.9, No.1 (2018). doi:10.1038/s41467-018-04859-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/44982
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Title
Rational Zika vaccine design via the modulation of antigen membrane anchors in chimpanzee adenoviral vectors
Author(s)
César López-Camacho
Peter Abbink
Rafael A. Larocca
Wanwisa Dejnirattisai
Michael Boyd
Alex Badamchi-Zadeh
Zoë R. Wallace
Jennifer Doig
Ricardo Sanchez Velazquez
Roberto Dias Lins Neto
Danilo F. Coelho
Young Chan Kim
Claire L. Donald
Ania Owsianka
Giuditta De Lorenzo
Alain Kohl
Sarah C. Gilbert
Lucy Dorrell
Juthathip Mongkolsapaya
Arvind H. Patel
Gavin R. Screaton
Dan H. Barouch
Adrian V.S. Hill
Arturo Reyes-Sandoval
Peter Abbink
Rafael A. Larocca
Wanwisa Dejnirattisai
Michael Boyd
Alex Badamchi-Zadeh
Zoë R. Wallace
Jennifer Doig
Ricardo Sanchez Velazquez
Roberto Dias Lins Neto
Danilo F. Coelho
Young Chan Kim
Claire L. Donald
Ania Owsianka
Giuditta De Lorenzo
Alain Kohl
Sarah C. Gilbert
Lucy Dorrell
Juthathip Mongkolsapaya
Arvind H. Patel
Gavin R. Screaton
Dan H. Barouch
Adrian V.S. Hill
Arturo Reyes-Sandoval
Abstract
© 2018 The Author(s). Zika virus (ZIKV) emerged on a global scale and no licensed vaccine ensures long-lasting anti-ZIKV immunity. Here we report the design and comparative evaluation of four replication-deficient chimpanzee adenoviral (ChAdOx1) ZIKV vaccine candidates comprising the addition or deletion of precursor membrane (prM) and envelope, with or without its transmembrane domain (TM). A single, non-adjuvanted vaccination of ChAdOx1 ZIKV vaccines elicits suitable levels of protective responses in mice challenged with ZIKV. ChAdOx1 prME ΔTM encoding prM and envelope without TM provides 100% protection, as well as long-lasting anti-envelope immune responses and no evidence of in vitro antibody-dependent enhancement to dengue virus. Deletion of prM and addition of TM reduces protective efficacy and yields lower anti-envelope responses. Our finding that immunity against ZIKV can be enhanced by modulating antigen membrane anchoring highlights important parameters in the design of viral vectored ZIKV vaccines to support further clinical assessments.