Publication: Assessment of Two New Commercial Rapid Tests for Canine N-terminal Pro-B-type Natriuretic Peptide to Distinguish the Severity of Mitral Valve Disease in Dogs
10
Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
No. of Pages/File Size
12 page
Journal Title
Journal of Applied Animal Science
Volume
14
Issue
2
Start Page
9
End Page
20
Access Rights
open access
Rights
ผลงานนี้เป็นลิขสิทธิ์ของมหาวิทยาลัยมหิดล ขอสงวนไว้สำหรับเพื่อการศึกษาเท่านั้น ต้องอ้างอิงแหล่งที่มา ห้ามดัดแปลงเนื้อหา และห้ามนำไปใช้เพื่อการค้า
Rights Holder(s)
Faculty of Veterinary Science Mahidol University
Bibliographic Citation
Journal of Applied Animal Science. Vol.14, No.2 (Jul - Dec 2021), 9-20
Suggested Citation
ณัฐพล เรียงวิโรจน์กิจ, วลาสินี ศักดิ์คำดวง, Nattapon Riengvirodkij, Walasinee Sakcamduang Assessment of Two New Commercial Rapid Tests for Canine N-terminal Pro-B-type Natriuretic Peptide to Distinguish the Severity of Mitral Valve Disease in Dogs. Journal of Applied Animal Science. Vol.14, No.2 (Jul - Dec 2021), 9-20. 20. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/112255
Research Projects
Organizational Units
Authors
Journal Issue
JAAS Vol. 14 No. 2
(2564)
Thesis
Title
Assessment of Two New Commercial Rapid Tests for Canine N-terminal Pro-B-type Natriuretic Peptide to Distinguish the Severity of Mitral Valve Disease in Dogs
Alternative Title(s)
การประเมินชุดตรวจ N-terminal pro-B-type natriuretic peptide ในการแยกระดับความรุนแรงของโรคลิ้นหัวใจเสื่อมในสุนัข
Author's Affiliation
มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. โรงพยาบาลสัตว์ประศูอาทร
มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ภาควิชาเวชศาสตร์คลินิกและการสาธารณสุข
Mahidol University. Faculty of Veterinary Science. Prasu-Arthorn Veterinary Teaching Hospital
Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health
มหาวิทยาลัยมหิดล. คณะสัตวแพทยศาสตร์. ภาควิชาเวชศาสตร์คลินิกและการสาธารณสุข
Mahidol University. Faculty of Veterinary Science. Prasu-Arthorn Veterinary Teaching Hospital
Mahidol University. Faculty of Veterinary Science. Department of Clinical Sciences and Public Health
Abstract
N-terminal pro-B-type natriuretic peptide (NT-pro-BNP) is a potential cardiac biomarker released
primarily from ventricular myocytes. Previous studies in dogs suggest that the NT-proBNP assay can be used to
distinguish dogs with respiratory signs between congestive heart failure and primary respiratory illnesses.
The objective of this study is to determine and assess the clinical sensitivity and specificity of the two new NT-proBNP immunoassay tests for distinguishing dogs affected by myxomatous mitral valve disease (MMVD)
between with and without congestive heart failure (CHF), as well as between dogs with MMVD and normal dogs.
Thirty-seven dogs were enrolled in this study. Thirty-two dogs were measured by the NT-proBNP immunoassay
test (Bionote®), including 11 healthy dogs, 11 dogs with MMVD stage B2 (absence of CHF) and 10 dogs with
MMVD stage C (presence of CHF). Twenty-eight dogs were measured by the NT-proBNP rapid detection kit
(Dianotech®), including 6 healthy dogs, 7 dogs with MMVD stage B2 and 15 dogs with MMVD stage C.
The median NT-proBNP concentration in dogs with MMVD stage C was significantly higher than the
median NT-proBNP concentration in dogs with MMVD stage B2 and normal healthy dogs (p-value <0.001).
The Receiver operating characteristic (ROC) curve analysis showed that serum NT-proBNP concentration could
differentiate dogs with CHF signs (MMVD stage C) from dogs without CHF signs (normal healthy dogs and
MMVD stage B2. The area under the curve (AUC) was 0.932 and 0.928 for NT-proBNP Bionote® and Dianotech®
test, respectively. It also could discriminate dogs affected by MMVD with CHF (MMVD stage C2) from dogs
affected by MMVD without CHF (MMVD stage B2) with AUC of 0.818 and 0.867 for NT-proBNP Bionote® and
Dianotech® tests, respectively. In conclusion, the results suggested that serum NT-proBNP concentrations
tests from both companies could discriminate between dogs affected by CHF from those without CHF and normal
healthy dogs with reasonable accuracy.