Publication: Liquid chromatography tandem mass spectrometry method for simultaneous determination of losartan and its active metabolite in human plasma
Issued Date
2018-01-01
Resource Type
ISSN
25868470
25868195
25868195
Other identifier(s)
2-s2.0-85060157317
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Pharmaceutical Sciences Asia. Vol.45, No.4 (2018), 252-262
Suggested Citation
Jureeporn Sri-In, Jedsadaporn Prakobkijcharoen, Wannisa Thanakosai, Somruedee Chatsiricharoenkul, Weerawadee Chandranipapongse Liquid chromatography tandem mass spectrometry method for simultaneous determination of losartan and its active metabolite in human plasma. Pharmaceutical Sciences Asia. Vol.45, No.4 (2018), 252-262. doi:10.29090/psa.2018.04.018.0012 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/47116
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Liquid chromatography tandem mass spectrometry method for simultaneous determination of losartan and its active metabolite in human plasma
Other Contributor(s)
Abstract
© Faculty of Pharmacy, Mahidol University (Thailand) 2018. A highly selective, sensitive and reproducible liquid chromatography tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of losartan and its active metabolite (EXP3174) in human plasma, using candesartan as an internal standard (IS), was described. Chromatographic separation was carried out in the Luna HST 2.5μm C18 (50×3 mm.). The mobile phases were 0.05% formic acid and acetonitrile at a ratio of 3.3:6.7 (v/v). The mass spectrometry was operated in positive electrospray ionization and multiple reactions monitoring (MRM) mode. Liquid-liquid extraction (LLE) was employed with the mixture of ethylacetate and hexane at a ratio of 9:1 (v/v). The method was developed and fully validated according to the USFDA guidance. The limit of detection (LOD) of losartan and EXP3174 were 0.10 and 0.20 ng/mL, respectively and the lower limit of quantification (LLOQ) of both losartan and EXP3174 were 0.5 ng/mL. This method demonstrated good linearity (r 2 > 0.999) ranged from 0.5-2,500 ng/mL for both losartan and EXP3174. Accuracy and precision of the method were acceptable with high recovery of extraction. Neither anticoagulant nor matrix affected the analysis. This method was successfully applied to determine the concentrations of losartan and EXP3174 in human plasma in a bioequivalence study.