Publication: Development and application of SSR markers derived from Bauhinia Strychnifolia a semi-endemic plant in Thailand
Issued Date
2018-10-02
Resource Type
ISSN
15403580
10496475
10496475
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2-s2.0-85054165769
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Herbs, Spices and Medicinal Plants. Vol.24, No.4 (2018), 386-393
Suggested Citation
Supajit Sraphet, Pattra Chantravisut, Nattaya Srisawad, Nawarat Suksee, Duncan R. Smith, Kanokporn Triwitayakorn Development and application of SSR markers derived from Bauhinia Strychnifolia a semi-endemic plant in Thailand. Journal of Herbs, Spices and Medicinal Plants. Vol.24, No.4 (2018), 386-393. doi:10.1080/10496475.2018.1520775 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/46255
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Title
Development and application of SSR markers derived from Bauhinia Strychnifolia a semi-endemic plant in Thailand
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Abstract
© 2018, © 2018 Taylor & Francis Group, LLC. Bauhinia strychnifolia is a rare and semi-endemic plant in Thailand. Microsatellite-enriched libraries were constructed using (CT)15/(CAG)15 and (GT)15/(AAG)15 probes. Fourteen polymorphic loci produced a total of 104 alleles with a mean of 7.4 alleles per locus. The average expected and observed heterozygosity were 0.727 and 0.497, respectively. The polymorphism information content value ranged from 0.335 to 0.910. Nine microsatellite loci were successfully transferred across species to B. galpiniii, B. Siamensis, and B. tomentosa. The information obtained in this study will be useful in establishing a conservation strategy for B. strychnifolia in Thailand and for genetic studies of other Bauhinia sp.