Publication: Prime-boost vaccination with recombinant protein and adenovirus-vector expressing Plasmodium vivax circumsporozoite protein (CSP) partially protects mice against Pb/Pv sporozoite challenge
Issued Date
2018-12-01
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20452322
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2-s2.0-85040766696
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Mahidol University
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SCOPUS
Bibliographic Citation
Scientific Reports. Vol.8, No.1 (2018)
Suggested Citation
Tarsila Mendes De Camargo, Elisângela Oliveira De Freitas, Alba Marina Gimenez, Luciana Chagas Lima, Karina De Almeida Caramico, Kátia Sanches Françoso, Oscar Bruna-Romero, Chiara Andolina, François Nosten, Laurent Rénia, Hildegund C.J. Ertl, Ruth S. Nussenzweig, Victor Nussenzweig, Mauricio M. Rodrigues, Arturo Reyes-Sandoval, Irene S. Soares Prime-boost vaccination with recombinant protein and adenovirus-vector expressing Plasmodium vivax circumsporozoite protein (CSP) partially protects mice against Pb/Pv sporozoite challenge. Scientific Reports. Vol.8, No.1 (2018). doi:10.1038/s41598-017-19063-6 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/47501
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Title
Prime-boost vaccination with recombinant protein and adenovirus-vector expressing Plasmodium vivax circumsporozoite protein (CSP) partially protects mice against Pb/Pv sporozoite challenge
Author(s)
Tarsila Mendes De Camargo
Elisângela Oliveira De Freitas
Alba Marina Gimenez
Luciana Chagas Lima
Karina De Almeida Caramico
Kátia Sanches Françoso
Oscar Bruna-Romero
Chiara Andolina
François Nosten
Laurent Rénia
Hildegund C.J. Ertl
Ruth S. Nussenzweig
Victor Nussenzweig
Mauricio M. Rodrigues
Arturo Reyes-Sandoval
Irene S. Soares
Elisângela Oliveira De Freitas
Alba Marina Gimenez
Luciana Chagas Lima
Karina De Almeida Caramico
Kátia Sanches Françoso
Oscar Bruna-Romero
Chiara Andolina
François Nosten
Laurent Rénia
Hildegund C.J. Ertl
Ruth S. Nussenzweig
Victor Nussenzweig
Mauricio M. Rodrigues
Arturo Reyes-Sandoval
Irene S. Soares
Abstract
© 2018 The Author(s). Vaccine development against Plasmodium vivax malaria lags behind that for Plasmodium falciparum. To narrow this gap, we administered recombinant antigens based on P. vivax circumsporozoite protein (CSP) to mice. We expressed in Pichia pastoris two chimeric proteins by merging the three central repeat regions of different CSP alleles (VK210, VK247, and P. vivax-like). The first construct (yPvCSP-AllFL) contained the fused repeat regions flanked by N- and C-terminal regions. The second construct (yPvCSP-AllCT) contained the fused repeat regions and the C-terminal domain, plus RI region. Mice were vaccinated with three doses of yPvCSP in adjuvants Poly (I:C) or Montanide ISA720. We also used replication-defective adenovirus vectors expressing CSP of human serotype 5 (AdHu5) and chimpanzee serotype 68 (AdC68) for priming mice which were subsequently boosted twice with yPvCSP proteins in Poly (I:C) adjuvant. Regardless of the regime used, immunized mice generated high IgG titres specific to all CSP alleles. After challenge with P. berghei ANKA transgenic parasites expressing Pb/PvVK210 or Pb/PvVK247 sporozoites, significant time delays for parasitemia were observed in all vaccinated mice. These vaccine formulations should be clinically tried for their potential as protective universal vaccine against P. vivax malaria.