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Intracellular bacteria interfere with dendritic cell functions: Role of the type I interferon pathway

dc.contributor.authorLaurent Gorvelen_US
dc.contributor.authorJulien Textorisen_US
dc.contributor.authorRomain Banchereauen_US
dc.contributor.authorAmira Ben Amaraen_US
dc.contributor.authorWiwit Tantibhedhyangkulen_US
dc.contributor.authorKristin Von Bargenen_US
dc.contributor.authorMignane B. Kaen_US
dc.contributor.authorChristian Capoen_US
dc.contributor.authorEric Ghigoen_US
dc.contributor.authorJean Pierre Gorveen_US
dc.contributor.authorJean Louis Megeen_US
dc.contributor.otherAix Marseille Universiteen_US
dc.contributor.otherBaylor Institute for Immunology Researchen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-11-09T01:44:30Z
dc.date.available2018-11-09T01:44:30Z
dc.date.issued2014-06-10en_US
dc.description.abstractDendritic cells (DCs) orchestrate host defenses against microorganisms. In infectious diseases due to intracellular bacteria, the inefficiency of the immune system to eradicate microorganisms has been attributed to the hijacking of DC functions. In this study, we selected intracellular bacterial pathogens with distinct lifestyles and explored the responses of monocyte-derived DCs (moDCs). Using lipopolysaccharide as a control, we found that Orientia tsutsugamushi, the causative agent of scrub typhus that survives in the cytosol of target cells, induced moDC maturation, as assessed by decreased endocytosis activity, the ability to induce lymphocyte proliferation and the membrane expression of phenotypic markers. In contrast, Coxiella burnetii, the agent of Q fever, and Brucella abortus, the agent of brucellosis, both of which reside in vacuolar compartments, only partly induced the maturation of moDCs, as demonstrated by a phenotypic analysis. To analyze the mechanisms used by C. burnetii and B. abortus to alter moDC activation, we performed microarray and found that C. burnetii and B. abortus induced a specific signature consisting of TLR4, TLR3, STAT1 and interferon response genes. These genes were down-modulated in response to C. burnetii and B. abortus but up-modulated in moDCs activated by lipopolysaccharide and O. tsutsugamushi. This transcriptional alteration was associated with the defective interferon-β production. This study demonstrates that intracellular bacteria specifically affect moDC responses and emphasizes how C. burnetii and B. abortus interfere with moDC activation and the antimicrobial immune response. We believe that comparing infection by several bacterial species may be useful for defining new pathways and biomarkers and for developing new treatment strategies. © 2014 Gorvel et al.en_US
dc.identifier.citationPLoS ONE. Vol.9, No.6 (2014)en_US
dc.identifier.doi10.1371/journal.pone.0099420en_US
dc.identifier.issn19326203en_US
dc.identifier.other2-s2.0-84902595133en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/33010
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84902595133&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleIntracellular bacteria interfere with dendritic cell functions: Role of the type I interferon pathwayen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84902595133&origin=inwarden_US

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