Publication: Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts
Issued Date
2011-05-01
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0145305X
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2-s2.0-79952448588
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Mahidol University
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SCOPUS
Bibliographic Citation
Developmental and Comparative Immunology. Vol.35, No.5 (2011), 545-553
Suggested Citation
Supawadee Pata, Nualyai Yaraksa, Sakda Daduang, Yosapong Temsiripong, Jisnuson Svasti, Tomohiro Araki, Sompong Thammasirirak Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts. Developmental and Comparative Immunology. Vol.35, No.5 (2011), 545-553. doi:10.1016/j.dci.2010.12.011 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11558
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Title
Characterization of the novel antibacterial peptide Leucrocin from crocodile (Crocodylus siamensis) white blood cell extracts
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Abstract
Four novel antibacterial peptides, Leucrocin I-IV from Siamese crocodile white blood cell extracts were purified by reverse phase high performance liquid chromatography (RP-HPLC). Leucrocins exhibit strong antibacterial activity towards Staphylococcus epidermidis, Salmonella typhi and Vibrio cholerae. The peptides were 7-10 residues in length with different primary structure. The amino acid sequence of Leucrocin I is NGVQPKY with molecular mass around 806.99. Da and Leucrocin II is NAGSLLSGWG with molecular mass around 956.3. Da. Further, the interaction between peptides and bacterial membranes as part of their killing mechanism was studied by fluorescence and electron microscopy. The outer membrane and cytoplasmic membrane was the target of action of Leucrocins as assayed in model membrane by release of β-galactosidase due to the membrane permeabilization. Finally, the hemolytic effect was tested against human red blood cell. Leucrocin I, III and IV showed less toxicity against human red blood cells than Leucrocin II. © 2010 Elsevier Ltd.