RT-LAMP detection of shrimp Taura syndrome virus (TSV) by combination with a nanogold-oligo probe

Abstract

© 2013 John Wiley & Sons Ltd. This study describes the application and optimization of a newly developed specific gold nanoparticle (AuNP) visual detection method to detect a reverse transcription loop-mediated isothermal amplification (RT-LAMP) product of Taura syndrome virus (TSV) in shrimp with simple and cheaper in comparison to conventional gel electrophoresis. Briefly, the visual detection method is based on prevention of a salt induced DNA-labelled AuNP colour change from red to blue-purple due to hybridization with complementary DNA amplicons that arise from a positive TSV RT-LAMP reaction. RT-LAMP combined with visual amplicon detection using the DNA-labelled AuNP-probe had high sensitivity and the probe did not cross react with amplicons obtained using specific LAMP methods with other shrimp viruses. The advantages of this assay include simplicity, short analysis time and low-cost, suitable for field laboratory applications.