Publication: Genetic instability in cervical cancer detected by arbitrarily primed polymerase chain reaction
Issued Date
2007-01-01
Resource Type
ISSN
2476762X
15137368
15137368
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2-s2.0-34547811581
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Cancer Prevention. Vol.8, No.1 (2007), 109-122
Suggested Citation
Rujinee Paditaporn, Suda Riengrojpitak, Phaibul Punyarit, Sangdean Chindavijak, Anant Karalak, Songsak Petmitr Genetic instability in cervical cancer detected by arbitrarily primed polymerase chain reaction. Asian Pacific Journal of Cancer Prevention. Vol.8, No.1 (2007), 109-122. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24282
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Title
Genetic instability in cervical cancer detected by arbitrarily primed polymerase chain reaction
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Abstract
The genetic instability in 54 Thai cervical cancer tissues were analyzed by Arbitrarily Primed Polymerase Chain Reaction (AP-PCR). The band alterations produced from 54 arbitrary primers were compared between the DNA finger printing from the patients and their corresponding normal cervical tissues. Results revealed 7 arbitrary primers provided DNA alteration patterns. Of these, an allelic loss in tumor DNA was found in DNA fingerprinting obtained from primers F-2 (64.8%), F-11 (68.5%), U-8 (51.9%), AE-3 (75.9%), AE-11 (53.7%), respectively. Moreover, DNA amplification was exhibited in patterns with primers B-12 (42.6%), J-16 (24.1%) and U-8 (70.4%). When genetic instability was investigated for associations with clinicopathological features, only the DNA amplified fragment with primer U-8 was significantly associated with stage II (P=0.030). Likewise, allelic loss amplified from arbitrary primer AE-3 showed significantly associate with age lower than 50 years old (P=0.003). Our findings suggest that the DNA alteration fragments produced from arbitrary primers of U-8 and AE-11 might be relevant to the pathogenesis of cervical cancer in Thai patients.