Publication: Development of immortalized hepatocyte-like cells from hMSCs
Issued Date
2012-01-20
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ISSN
10643745
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2-s2.0-84855910106
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Mahidol University
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SCOPUS
Bibliographic Citation
Methods in Molecular Biology. Vol.826, (2012), 73-87
Suggested Citation
Adisak Wongkajornsilp, Khanit Sa-Ngiamsuntorn, Suradej Hongeng Development of immortalized hepatocyte-like cells from hMSCs. Methods in Molecular Biology. Vol.826, (2012), 73-87. doi:10.1007/978-1-61779-468-1_7 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13821
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Title
Development of immortalized hepatocyte-like cells from hMSCs
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Abstract
Clones of hepatocyte-like cells were reproducibly generated from human mesenchymal stem cells immortalized with a combined transduction of both Bmi-1 and TERT genes. These hepatocyte-like cells contained selective markers and several functional properties of hepatocytes, yet still carried proliferative potential. These cells had cuboidal morphology and arranged themselves as cord-like structure in culture. The cloned cells deposited glycogen and actively synthesized albumin. The basal expressions of CYP450 isozymes was observed, albeit only 10-20% that of primary hepatocytes. These expressions were promptly increased upon the addition of rifampicin, a known enzyme inducer. These hepatocyte-like cells may serve as a close alternative to the use of primary hepatocytes for in vitro studies. © 2012 Springer Science+Business Media, LLC.