Publication: Evaluation of lymphocyte apoptosis in patients with oral cancer
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Issued Date
2020-09-07
Resource Type
ISSN
16787765
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2-s2.0-85091264391
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of applied oral science : revista FOB. Vol.28, (2020), e20200124
Suggested Citation
Fardeela Bin-Alee, Areeya Arayataweegool, Supranee Buranapraditkun, Patnarin Mahattanasakul, Napadon Tangjaturonrasme, Apiwat Mutirangura, Nakarin Kitkumthorn Evaluation of lymphocyte apoptosis in patients with oral cancer. Journal of applied oral science : revista FOB. Vol.28, (2020), e20200124. doi:10.1590/1678-7757-2020-0124 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/59047
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Title
Evaluation of lymphocyte apoptosis in patients with oral cancer
Abstract
OBJECTIVES: To evaluate apoptotic levels of peripheral blood mononuclear cells (PBMCs) and apoptotic regulatory proteins (Bax and Bcl-2) in lymphocyte subsets of oral cancer (OC) patients and healthy controls (HC). METHODOLOGY: The percentage of apoptotic cells and lymphocyte counts were measured in the first cohort using PBMCs obtained from 23 OC patients and 6 HC. In the second cohort, (OC, 33; HC, 13), the mean fluorescence intensity (MFI) of Bax and Bcl-2 in CD19+ B, CD4+ T, CD8+ T, and CD16+56+ natural killer (NK) cells was determined via flow cytometry. RESULTS: The percentage of apoptotic cells was higher in the PBMCs of OC patients than in HC patients, particularly in patients with stage IV cancer (p<0.05). However, lymphocyte counts were significantly lower in stage IV patients (p<0.05). NK CD19+ B and CD16+56+ cell counts were significantly lower in OC patients compared with HC patients (p<0.001 and p<0.01, respectively), but CD4+ T cells were interestingly significantly higher in OC patients (p<0.001). While Bax MFI was slightly higher, Bcl-2 MFI was significantly lower for all four lymphocyte subsets in OC samples, particularly in stage IV patients, when compared with HC. Consequently, Bax/Bcl-2 ratios showed an upward trend from HC to OC patients, particularly those in stage IV. We found similar trends in Bax and Bcl-2 MFI for tumor stage, tumor size, and lymph node involvement. CONCLUSIONS: The increased lymphocyte apoptosis in stage IV OC patients may be related to higher Bax levels and lower Bcl-2 levels. The Bax/Bcl-2 ratio in lymphocytes may be useful to determine the prognosis of OC patients, and could be considered a mean for supportive treatment in the future.