Publication: Effects of Arsenic and Iron on the Community and Abundance of Arsenite-Oxidizing Bacteria in an Arsenic-Affected Groundwater Aquifer
Issued Date
2021-04-01
Resource Type
ISSN
14320991
03438651
03438651
Other identifier(s)
2-s2.0-85101807944
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Current Microbiology. Vol.78, No.4 (2021), 1324-1334
Suggested Citation
Phurinat Pipattanajaroenkul, Srilert Chotpantarat, Teerasit Termsaithong, Prinpida Sonthiphand Effects of Arsenic and Iron on the Community and Abundance of Arsenite-Oxidizing Bacteria in an Arsenic-Affected Groundwater Aquifer. Current Microbiology. Vol.78, No.4 (2021), 1324-1334. doi:10.1007/s00284-021-02418-8 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/77304
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Effects of Arsenic and Iron on the Community and Abundance of Arsenite-Oxidizing Bacteria in an Arsenic-Affected Groundwater Aquifer
Other Contributor(s)
Abstract
Arsenic (As) contamination of groundwater aquifers is a global environmental problem, especially in South and Southeast Asian regions, and poses a risk to human health. Arsenite-oxidizing bacteria that transform As(III) to less toxic As(V) can be potentially used as a groundwater As remediation strategy. This study aimed to examine the community and abundance of arsenite-oxidizing bacteria in groundwater with various As concentrations from Rayong Province, Thailand using PCR-cloning-sequencing and quantitative PCR (qPCR) of catalytic subunit of arsenite oxidase gene (aioA). Key factors influencing their community and abundance were also identified. The results demonstrated that arsenite-oxidizing bacteria retrieved from groundwater were phylogenetically related to Betaproteobacteria and Alphaproteobacteria. The aioA gene abundances ranged from 8.6 × 101 to 1.1 × 104 copies per ng of genomic DNA, accounting for 0.16–1.37% of the total 16S rRNA bacterial gene copies. Although the abundance of arsenite-oxidizing bacteria in groundwater was low, groundwater with As(III) dominance likely promoted their abundance which possibly played an important role in chemolithoautotrophic oxidation of As(III) to As(V). Fe and As(III) were the major environmental factors influencing the community and abundance of arsenite-oxidizing bacteria. The knowledge gained from this study can be used to further contribute to the development of bioremediation strategies for As removal from groundwater resources.