Publication: Complement alternative pathway genetic variation and Dengue infection in the Thai population
Issued Date
2013-11-01
Resource Type
ISSN
13652249
00099104
00099104
Other identifier(s)
2-s2.0-84885162537
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinical and Experimental Immunology. Vol.174, No.2 (2013), 326-334
Suggested Citation
R. Kraivong, S. Vasanawathana, W. Limpitikul, P. Malasit, N. Tangthawornchaikul, M. Botto, G. R. Screaton, J. Mongkolsapaya, M. C. Pickering Complement alternative pathway genetic variation and Dengue infection in the Thai population. Clinical and Experimental Immunology. Vol.174, No.2 (2013), 326-334. doi:10.1111/cei.12184 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31849
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Title
Complement alternative pathway genetic variation and Dengue infection in the Thai population
Abstract
Dengue disease is a mosquito-borne infection caused by Dengue virus. Infection may be asymptomatic or variably manifest as mild Dengue fever (DF) to the most severe form, Dengue haemorrhagic fever (DHF). Mechanisms that influence disease severity are not understood. Complement, an integral component of the immune system, is activated during Dengue infection and the degree of activation increases with disease severity. Activation of the complement alternative pathway is influenced by polymorphisms within activation (factor B rs12614/rs641153, C3 rs2230199) and regulatory [complement factor H (CFH) rs800292] proteins, collectively termed a complotype. Here, we tested the hypothesis that the complotype influences disease severity during secondary Dengue infection. In addition to the complotype, we also assessed two other disease-associated CFH polymorphisms (rs1061170, rs3753394) and a structural polymorphism within the CFH protein family. We did not detect any significant association between the examined polymorphisms and Dengue infection severity in the Thai population. However, the minor allele frequencies of the factor B and C3 polymorphisms were less than 10%, so our study was not sufficiently powered to detect an association at these loci. We were also unable to detect a direct interaction between CFH and Dengue NS1 using both recombinant NS1 and DV2-infected culture supernatants. We conclude that the complotype does not influence secondary Dengue infection severity in the Thai population. © 2013 The Authors. Clinical and Experimental Immunology published by John Wiley & Sons Ltd on behalf of British Society. for Immunology.