Publication: Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting.
Accepted Date
2014-10-29
Issued Date
2015-01
Copyright Date
2015
Resource Type
Language
eng
ISSN
1088-9051 (printed)
1549-5469 (electronic)
1549-5469 (electronic)
Rights Holder(s)
Genome research
Bibliographic Citation
Tong SY, Holden MT, Nickerson EK, Cooper BS, Köser CU, Cori A. et al. Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting. Genome Res. 2015 Jan;25(1):111-8.
Suggested Citation
Tong, Steven Y.C., Holden, Matthew T.G., Nickerson, Emma K., Cooper, Ben S., Köser, Claudio U., Cori, Anne, Jombart, Thibaut, Cauchemez, Simon, Fraser, Christophe, Vanaporn Wuthiekanun, วรรณพร วุฒิเอกอนันต์, Janjira Thaipadungpanit, จันทร์จิรา ไทยผดุงพานิช, Maliwan Hongsuwan, มะลิวัลย์ หงษ์สุวรรณ, Day, Nicholas P., Direk Limmathurotsakul, ดิเรก ลิ้มมธุรสกุล, Parkhill, Julian, Peacock, Sharon J. Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting.. Tong SY, Holden MT, Nickerson EK, Cooper BS, Köser CU, Cori A. et al. Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting. Genome Res. 2015 Jan;25(1):111-8.. doi:10.1101/gr.174730.114. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/849
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Title
Genome sequencing defines phylogeny and spread of methicillin-resistant Staphylococcus aureus in a high transmission setting.
Author(s)
Tong, Steven Y.C.
Holden, Matthew T.G.
Nickerson, Emma K.
Cooper, Ben S.
Köser, Claudio U.
Cori, Anne
Jombart, Thibaut
Cauchemez, Simon
Fraser, Christophe
Vanaporn Wuthiekanun
วรรณพร วุฒิเอกอนันต์
Janjira Thaipadungpanit
จันทร์จิรา ไทยผดุงพานิช
Maliwan Hongsuwan
มะลิวัลย์ หงษ์สุวรรณ
Day, Nicholas P.
Direk Limmathurotsakul
ดิเรก ลิ้มมธุรสกุล
Parkhill, Julian
Peacock, Sharon J.
Holden, Matthew T.G.
Nickerson, Emma K.
Cooper, Ben S.
Köser, Claudio U.
Cori, Anne
Jombart, Thibaut
Cauchemez, Simon
Fraser, Christophe
Vanaporn Wuthiekanun
วรรณพร วุฒิเอกอนันต์
Janjira Thaipadungpanit
จันทร์จิรา ไทยผดุงพานิช
Maliwan Hongsuwan
มะลิวัลย์ หงษ์สุวรรณ
Day, Nicholas P.
Direk Limmathurotsakul
ดิเรก ลิ้มมธุรสกุล
Parkhill, Julian
Peacock, Sharon J.
Corresponding Author(s)
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial
infection. Whole-genome sequencing of MRSA has been used to define phylogeny and
transmission in well-resourced healthcare settings, yet the greatest burden of
nosocomial infection occurs in resource-restricted settings where barriers to
transmission are lower. Here, we study the flux and genetic diversity of MRSA on
ward and individual patient levels in a hospital where transmission was common.
We repeatedly screened all patients on two intensive care units for MRSA carriage
over a 3-mo period. All MRSA belonged to multilocus sequence type 239 (ST 239).
We defined the population structure and charted the spread of MRSA by sequencing
79 isolates from 46 patients and five members of staff, including the first
MRSA-positive screen isolates and up to two repeat isolates where available.
Phylogenetic analysis identified a flux of distinct ST 239 clades over time in
each intensive care unit. In total, five main clades were identified, which
varied in the carriage of plasmids encoding antiseptic and antimicrobial
resistance determinants. Sequence data confirmed intra- and interwards
transmission events and identified individual patients who were colonized by more
than one clade. One patient on each unit was the source of numerous transmission
events, and deep sampling of one of these cases demonstrated colonization with a
"cloud" of related MRSA variants. The application of whole-genome sequencing and
analysis provides novel insights into the transmission of MRSA in under-resourced
healthcare settings and has relevance to wider global health.