Publication: HIV-1 subtyping using gag/env heteroduplex mobility assay and peptide enzyme-linked immunosorbent assay
Issued Date
2005-06-01
Resource Type
ISSN
0125877X
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2-s2.0-27544447901
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Allergy and Immunology. Vol.23, No.2-3 (2005), 143-152
Suggested Citation
Wantanee Simparak, Uraiwan Kositanont, Ruengpung Sutthent, Punneeporn Wasinrapee, Thanyanan Chaowanachan, Chantapong Wasi HIV-1 subtyping using gag/env heteroduplex mobility assay and peptide enzyme-linked immunosorbent assay. Asian Pacific Journal of Allergy and Immunology. Vol.23, No.2-3 (2005), 143-152. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/16583
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Title
HIV-1 subtyping using gag/env heteroduplex mobility assay and peptide enzyme-linked immunosorbent assay
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Abstract
Two HIV-1 subtypes have accounted for virtually all infections in Thailand: subtype B′, found mainly in injection drug users (IDUs), and CRF01Æ (initially subtype E), found in over 90% of sexually infected persons and increasingly in IDUs in recent years. During 1997-1998, 227 blood samples were collected from HIV-1 infected individuals consisting of 92 mothers, 35 children and 100 IDUs. The blood samples were subtyped by heteroduplex mobility assay (HMA) and peptide enzyme-linked immunosorbent assay (PEIA). Using gag and env HMA, CRF01Æ and subtype B′ accounted for 96-97% and 3-4% of both the mothers and the children, respectively. In the IDU group, 10% of the plasma samples could only be performed by gag HMA and gave the result as CRF01Æ. CRF01Æ and subtype B′ using PEIA accounted for 67% and 33% of the IDUs. There was 100% concordance of the results between gag HMA and env HMA. Ninety-five percentages of concordant results were observed between HMA and PEIA. Of the 6/134 (5%) subjects with discordant results, nucleotide sequencing, used as a gold standard, confirmed the HMA result. In this study, HIV-1 was successfully genotyped by HMA and PEIA. However, a comparison of the subtyping results between HMA and PEIA revealed that HMA was slightly more accurate than PEIA.