Publication: Glucose-6-phosphate dehydrogenase (G6PD) mutations and haemoglobinuria syndrome in the vietnamese population
Issued Date
2009-08-20
Resource Type
ISSN
14752875
Other identifier(s)
2-s2.0-68749119762
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Malaria Journal. Vol.8, No.1 (2009)
Suggested Citation
Nguyen Thi Hue, Jean Paul Charlieu, Tran Thi Hong Chau, Nick Day, Jeremy J. Farrar, Tran Tinh Hien, Sarah J. Dunstan Glucose-6-phosphate dehydrogenase (G6PD) mutations and haemoglobinuria syndrome in the vietnamese population. Malaria Journal. Vol.8, No.1 (2009). doi:10.1186/1475-2875-8-152 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27669
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Glucose-6-phosphate dehydrogenase (G6PD) mutations and haemoglobinuria syndrome in the vietnamese population
Other Contributor(s)
Abstract
Background. In Vietnam the blackwater fever syndrome (BWF) has been associated with malaria infection, quinine ingestion and G6PD deficiency. The G6PD variants within the Vietnamese Kinh contributing to the disease risk in this population, and more generally to haemoglobinuria, are currently unknown. Method. Eighty-two haemoglobinuria patients and 524 healthy controls were screened for G6PD deficiency using either the methylene blue reduction test, the G-6-PDH kit or the micro-methaemoglobin reduction test. The G6PD gene variants were screened using SSCP combined with DNA sequencing in 82 patients with haemoglobinuria, and in 59 healthy controls found to be G6PD deficient. Results. This study confirmed that G6PD deficiency is strongly associated with haemoglobinuria (OR = 15, 95% CI [7.7 to 28.9], P < 0.0001). Six G6PD variants were identified in the Vietnamese population, of which two are novel (Vietnam1 [Glu- 3- Lys] and Vietnam2 [Phe-66-Cys]). G6PD Viangchan [Val-291- Met], common throughout south-east Asia, accounted for 77% of the variants detected and was significantly associated with haemoglobinuria within G6PD-deficient ethnic Kinh Vietnamese (OR = 5.8 95% CI [114-55.4], P = 0.022). Conclusion. The primary frequency of several G6PD mutations, including novel mutations, in the Vietnamese Kinh population are reported and the contribution of G6PD mutations to the development of haemoglobinuria are investigated. © 2009 Hue et al; licensee BioMed Central Ltd.