Publication: Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits
Issued Date
2012-08-01
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ISSN
13474421
13891723
13891723
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2-s2.0-84863185814
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Bioscience and Bioengineering. Vol.114, No.2 (2012), 144-149
Suggested Citation
Choowong Auesukaree, Preeyaporn Koedrith, Pornpon Saenpayavai, Thipa Asvarak, Suthee Benjaphokee, Minetaka Sugiyama, Yoshinobu Kaneko, Satoshi Harashima, Chuenchit Boonchird Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits. Journal of Bioscience and Bioengineering. Vol.114, No.2 (2012), 144-149. doi:10.1016/j.jbiosc.2012.03.012 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13653
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Title
Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits
Abstract
For industrial applications, fermentation of ethanol at high temperature offers advantages such as reduction in cooling costs, reduced risk of microbial contamination and higher efficiency of fermentation processes including saccharification and continuous ethanol stripping. Three thermotolerant Saccharomyces cerevisiae isolates (C3723, C3751 and C3867) from Thai fruits were capable of growing and producing 38 g/L ethanol up to 41°C. Based on genetic analyses, these isolates were prototrophic and homothallic, with dominant homothallic and thermotolerant phenotypes. After short-term (30 min) and long-term (12 h) exposure at 37°C, expression levels increased for the heat stress-response genes HSP26, SSA4, HSP82, and HSP104 encoding the heat shock proteins small HSP, HSP70, HSP90 and the HSP100 family, respectively. In isolates C3723 and C3867, expression was significantly higher than that in reference isolates W303 and TISTR5606 for TPS1 encoding trehalose-6-phosphate synthase, NTH1 encoding neutral trehalase and GSY1 encoding glycogen synthase. The results suggested that continuous high expression of heat stress-response genes was important for the long-term, heat stress tolerance of these thermotolerant isolates. © 2012 The Society for Biotechnology, Japan.