Publication: Tick-borne encephalitis virus infection of cultured mouse macrophages
Issued Date
2009-09-01
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ISSN
03005526
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2-s2.0-69049115411
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Mahidol University
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SCOPUS
Bibliographic Citation
Intervirology. Vol.52, No.5 (2009), 283-290
Suggested Citation
Arunee Ahantarig, Daniel Rüžek, Marie Vancová, Anna Janowitz, Hana Št'Astná, Martina Tesařová, Libor Grubhoffer Tick-borne encephalitis virus infection of cultured mouse macrophages. Intervirology. Vol.52, No.5 (2009), 283-290. doi:10.1159/000235741 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27663
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Title
Tick-borne encephalitis virus infection of cultured mouse macrophages
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Abstract
The interactions of tick-borne encephalitis virus (TBEV) with mouse macrophages were studied at the electron microscopic level. The cultured mouse macrophages were sensitive to infection with TBEV strain Hypr (a highly neuroinvasive and neurovirulent strain for laboratory mice) and produced relatively high virus titers. However, these macrophage cells remained morphologically inactivated. Viral particles were located mainly in the ER but were also present in other exocytic compartments. No virus production was observed in cells infected with the attenuated, non-neuroinvasive TBEV strain 263. In this case, the infection led to a clear morphological activation of the macrophages. In conclusion, the virus replication process in mouse macrophage cells might be different from that in other mammalian cell lines since the smooth membrane structures, which are thought to be the sites for flavivirus replication, were not observed. Moreover, different TBEV strains exhibited a different interaction with the host macrophages. The inability of strain 263 to replicate in mouse macrophages as the first site of significant viral replication in vivo could be associated with the inability of this strain to establish a serious infection in mice. © 2009 S. Karger AG, Basel.