Publication: Successful propagation of shrimp yellow head virus in immortal mosquito cells
1
Issued Date
2010-05-18
Resource Type
ISSN
16161580
01775103
01775103
DOI
Other identifier(s)
2-s2.0-77954123332
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Diseases of Aquatic Organisms. Vol.90, No.1 (2010), 77-83
Suggested Citation
Warachin Gangnonngiw, Nipaporn Kanthong, Timothy W. Flegel Successful propagation of shrimp yellow head virus in immortal mosquito cells. Diseases of Aquatic Organisms. Vol.90, No.1 (2010), 77-83. doi:10.3354/dao02220 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28503
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Successful propagation of shrimp yellow head virus in immortal mosquito cells
Abstract
Research on crustacean viruses is hampered by the lack of continuous cell lines susceptible to them. To overcome this problem, we previously challenged immortal mosquito and lepidopteran cell lines with shrimp yellow head virus (YHV), followed by serial, split-passage of whole cells, and showed that this produced cells that persistently expressed YHV antigens. To determine whether such insect cultures positive for YHV antigens could be used to infect shrimp Penaeus monodon with YHV, culture supernatants and whole-cell homogenates were used to challenge shrimp by injection. Shrimp injected with culture supernatants could not be infected. However, shrimp injectionchallenged with whole-cell homogenates from Passage 5 (early-passage) of such cultures died with histological and clinical signs typical for yellow head disease (YHD), while homogenates of mock-passaged, YHV-challenged cells did not. By contrast, shrimp challenged with cell homogenates of late-passage cultures became infected with YHV, but survived, suggesting that YHV attenuation had occurred during its long-term serial passage in insect cells. Thus, YHV could be propagated successfully in C6/36 mosquito cells and used at low passage numbers as a source of inoculum to initiate lethal infections in shrimp. This partially solves the problem of lack of continuous shrimp cell lines for cultivation of YHV. © Inter-Research 2010.