Publication: Novel salivary gland allergens from tropical mosquito species and IgE reactivity in allergic patients
Issued Date
2020-02-01
Resource Type
ISSN
19394551
Other identifier(s)
2-s2.0-85079400366
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
World Allergy Organization Journal. Vol.13, No.2 (2020)
Suggested Citation
Anunya Opasawatchai, Watchareewan Yolwong, Walairat Thuncharoen, Nanthicha Inrueangsri, Sulak Itsaradisaikul, Cherapat Sasisakulporn, Wanlapa Jotikasthira, Oranart Matangkasombut, Onrapak Reamtong, Wiparat Manuyakorn, Wisuwat Songnuan, Ponpan Matangkasombut Novel salivary gland allergens from tropical mosquito species and IgE reactivity in allergic patients. World Allergy Organization Journal. Vol.13, No.2 (2020). doi:10.1016/j.waojou.2020.100099 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/53698
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Novel salivary gland allergens from tropical mosquito species and IgE reactivity in allergic patients
Abstract
© 2020 The Authors Background: Mosquito allergy is common in tropical countries but remains under-diagnosed. This may be due to the lack of knowledge and diagnostic tools for tropical mosquito allergens. Objective: We aimed to characterize allergens from tropical mosquito species and investigate IgE reactivity in mosquito-allergic patients to the salivary gland proteins from these mosquitoes. Methods: Salivary gland extract (SGE) from 4 mosquito species, highly distributed in the tropics, including Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles dirus b, were studied. SGE-specific IgE and IgG ELISA were developed, and serum from 64 mosquito-allergic and 22 non-allergic healthy control subjects was assayed. Further investigations using IgE-immunoblots followed by mass spectrometry analysis were performed to identify and characterize allergens from each species. Results: Mosquito-allergic subjects have detectable serum IgE to SGE derived from local mosquito species, while the IgE levels to Aedes communis using commercially available ELISA were mostly minimal. IgE-immunoblot analysis and mass spectrometry identified 5 novel mosquito allergens from A. albopictus (Aed al 2, Aed al 3), C. quinquefasciatus (Cul q 2.01, Cul q 3), and A. dirus b (Ano d 2). Interestingly, 4 of the 5 new allergens belong to the D7 protein family. Conclusions & clinical relevance: Five novel allergens from 3 tropical mosquito species were characterized. The majority of mosquito-allergic subjects who live in the tropics have IgE reactivity to these allergens. Our study paves the way for the development of diagnostic tests, component-resolved diagnostics, and future immunotherapy for mosquito allergy in tropical countries.