Publication: Biodegradation of organophosphate pesticide using recombinant cyanobacteria with surface- and intracellular-expressed organophosphorus hydrolase
Issued Date
2008-05-28
Resource Type
ISSN
17388872
10177825
10177825
Other identifier(s)
2-s2.0-50249181034
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Microbiology and Biotechnology. Vol.18, No.5 (2008), 946-951
Suggested Citation
Wipa Chungjatupornchai, Sirirat Fa-Aroonsawat Biodegradation of organophosphate pesticide using recombinant cyanobacteria with surface- and intracellular-expressed organophosphorus hydrolase. Journal of Microbiology and Biotechnology. Vol.18, No.5 (2008), 946-951. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/18917
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Biodegradation of organophosphate pesticide using recombinant cyanobacteria with surface- and intracellular-expressed organophosphorus hydrolase
Author(s)
Other Contributor(s)
Abstract
The opd gene, encoding organophosphorus hydrolase (OPH) from Flavobacterium sp. capable of degrading a wide range of organophosphate pesticides, was surface- and intracellular-expressed in Synechococcus PCC7942, a prime example of photoautotrophic cyanobacteria. OPH was displayed on the cyanobacterial cell surface using the truncated ice nucleation protein as an anchoring motif. A minor fraction of OPH was displayed onto the outermost surface of cyanobacterial cells, as verfied by immunostaining visualized under confocal laser scanning microscopy and OPH activity analysis; however, a substantial fraction of OPH was buried in the cell wall, as demonstrated by proteinase K and lysozyme treatments. The cyanobacterial outer membrane acts as a substrate (paraoxon) diffusion barrier affecting whole-cell biodegradation efficiency. After freeze-thaw treatment, permeabilized whole cells with intracellular-expressed OPH exhibited 14-fold higher bioconversion efficiency (Vmax/Km) than that of cells with surface-expressed OPH. As cyanobacteria have simple growth requirements and are inexpensive to maintain, expression of OPH in cyanobacteria may lead to the development of a low-cost and low-maintenance biocatalyst that is useful for detoxification of organophosphate pesticides. © The Korean Society for Microbiology and Biotechnology.