Publication: Salsolinol, a dopamine-derived tetrahydroisoquinoline, induces cell death by causing oxidative stress in dopaminergic SH-SY5Y cells, and the said effect is attenuated by metallothionein
Issued Date
2004-04-16
Resource Type
ISSN
00068993
Other identifier(s)
2-s2.0-1642363224
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Mahidol University
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SCOPUS
Bibliographic Citation
Brain Research. Vol.1005, No.1-2 (2004), 67-76
Suggested Citation
Sawitri Wanpen, Piyarat Govitrapong, Shaik Shavali, Patcharee Sangchot, Manuchair Ebadi Salsolinol, a dopamine-derived tetrahydroisoquinoline, induces cell death by causing oxidative stress in dopaminergic SH-SY5Y cells, and the said effect is attenuated by metallothionein. Brain Research. Vol.1005, No.1-2 (2004), 67-76. doi:10.1016/j.brainres.2004.01.054 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/21197
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Title
Salsolinol, a dopamine-derived tetrahydroisoquinoline, induces cell death by causing oxidative stress in dopaminergic SH-SY5Y cells, and the said effect is attenuated by metallothionein
Abstract
The endogenous neurotoxin, 1-methyl-6,7-dihydroxy-1,2,3,4- tetrahydroisoquinoline (salsolinol), has been considered a potential neurotoxin in the etiology of Parkinson's disease (PD). Salsolinol and N-methyl(R)- salsolinol were identified in the brains and cerebrospinal fluid (CSF) of PD patients. Oxidative stress is known to be one of the major contributing factors in the cascade that may finally leads to the cell death in PD. The present study was undertaken to understand the role of salsolinol in oxidative-mediated neuronal toxicity in dopaminergic SH-SY5Y cells, and the neuroprotective effects of metallothionein (MT) against salsolinol toxicity in MT overexpressing (MTtrans) fetal mesencephalic cells. Salsolinol increased the production of reactive oxygen species (ROS) and significantly decreased glutathione (GSH) levels and cell viability in SH-SY5Y cells. Salsolinol also decreased intracellular ATP levels and induced nuclear condensation in these cells. Salsolinol-induced depletion in cell viability was completely prevented by N-acetylcysteine in SH-SY5Y cells, and also prevented by MT in MTtransfetal mesencephalic cells compared to controlwtcells. The extent of nuclear condensation and caspase activation was also less in MTtranscells than controlwtcells. These results suggest that salsolinol causes oxidative stress by decreasing the levels of GSH and by increasing ROS production, and these events may lead to the death of dopaminergic cell. Furthermore, MT overexpression may protect dopaminergic neurons against salsolinol-induced neurotoxicity, most probably by the inhibition of oxidative stress and apoptotic pathways including caspase-3 activation. © 2004 Elsevier B.V. All rights reserved.