Publication: Multiple displacement amplification for malaria parasite DNA
Issued Date
2009-02-01
Resource Type
ISSN
00223395
Other identifier(s)
2-s2.0-64549138851
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Parasitology. Vol.95, No.1 (2009), 253-255
Suggested Citation
Y. Wang, S. Nair, F. Nosten, T. J C Anderson Multiple displacement amplification for malaria parasite DNA. Journal of Parasitology. Vol.95, No.1 (2009), 253-255. doi:10.1645/GE-1706.1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27058
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Multiple displacement amplification for malaria parasite DNA
Author(s)
Abstract
Multiple displacement amplification (MDA) using Phi29 has proved to be an efficient, high-fidelity method for whole genome amplification in many organisms. This project was designed to evaluate this approach for use with the malaria parasite Plasmodium falciparum. In particular, we were concerned that the AT richness and presence of contaminating human DNA could limit efficiency of MDA in this system. We amplified 60 DNA samples using phi29 and scored 14 microsatellites, 9 single-nucleotide polymorphisms (SNPs), and gene copy number at GTP-cyclohydrolase I both before and after MDA. We observed 100% concordance in 829 microsatellite genotypes and in 499 SNP genotypes. Furthermore, copy number estimates for the GTP-cyclohydrolase I gene were correlated (r 2 0.67) in pre- and postamplification samples. These data confirm that MDA permits scoring of a range of different types of polymorphisms in P. falciparum malaria and can be used to extend the life of valuable DNA stocks. © 2009 American Society of Parasitologists.