Publication:
Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi

Issued Date

2009-12-01

Resource Type

Article

ISSN

00951137

DOI

10.1128/JCM.00811-09

Other identifier(s)

2-s2.0-71549116085

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of Clinical Microbiology. Vol.47, No.12 (2009), 4173-4175

Suggested Citation

Mallika Imwong, Naowarat Tanomsing, Sasithon Pukrittayakamee, Nicholas P J Day, Nicholas J. White, Georges Snounou Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi. Journal of Clinical Microbiology. Vol.47, No.12 (2009), 4173-4175. doi:10.1128/JCM.00811-09 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27858

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Title

Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi

Author(s)

Mallika Imwong
 Naowarat Tanomsing
 Sasithon Pukrittayakamee
 Nicholas P J Day
 Nicholas J. White
 Georges Snounou

Other Contributor(s)

Mahidol University
 Royal Institute
 Churchill Hospital
 Hopital Pitie Salpetriere
 Sorbonne Universite
 National University of Singapore, Faculty of Medicine

Abstract

The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes. Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Keyword(s)

Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/27858

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Scopus 2006-2010