Publication:
Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi

2

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2009-12-01

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Article

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00951137

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10.1128/JCM.00811-09

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2-s2.0-71549116085

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Mahidol University

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SCOPUS

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Journal of Clinical Microbiology. Vol.47, No.12 (2009), 4173-4175

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Mallika Imwong, Naowarat Tanomsing, Sasithon Pukrittayakamee, Nicholas P J Day, Nicholas J. White, Georges Snounou Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi. Journal of Clinical Microbiology. Vol.47, No.12 (2009), 4173-4175. doi:10.1128/JCM.00811-09 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/27858

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item.page.dc.title

Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi

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Mallika Imwong
 Naowarat Tanomsing
 Sasithon Pukrittayakamee
 Nicholas P J Day
 Nicholas J. White
 Georges Snounou

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Mahidol University
 Royal Institute
 Churchill Hospital
 Hopital Pitie Salpetriere
 Sorbonne Universite
 National University of Singapore, Faculty of Medicine

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The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes. Copyright © 2009, American Society for Microbiology. All Rights Reserved.

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Medicine

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https://repository.li.mahidol.ac.th/handle/123456789/27858

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