Publication: An Improved Method for Concentrating Rotavirus from Water Samples
Issued Date
2001-01-01
Resource Type
ISSN
00740276
Other identifier(s)
2-s2.0-0035431478
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Memorias do Instituto Oswaldo Cruz. Vol.96, No.6 (2001), 815-821
Suggested Citation
Leera Kittigul, Porntip Khamoun, Dusit Sujirarat, Fuangfa Utrarachkij, Kitja Chitpirom, Nattasai Chaichantanakit, Kanda Vathanophas An Improved Method for Concentrating Rotavirus from Water Samples. Memorias do Instituto Oswaldo Cruz. Vol.96, No.6 (2001), 815-821. doi:10.1590/S0074-02762001000600013 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/26861
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
An Improved Method for Concentrating Rotavirus from Water Samples
Other Contributor(s)
Abstract
A modified adsorption-elution method for the concentration of seeded rotavirus from water samples was used to determine various factors which affected the virus recovery. An enzyme-linked immunosorbent assay was used to detect the rotavirus antigen after concentration. Of the various eluents compared, 0.05M glycine, pH 11.5 gave the highest rotavirus antigen recovery using negatively charged membrane filtration whereas 2.9% tryptose phosphate broth containing 6% glycine; pH 9.0 was found to give the greatest elution efficiency when a positively charged membrane was used. Reconcentration of water samples by a speedVac concentrator showed significantly higher rotavirus recovery than polyethylene glycol precipitation through both negatively and positively charged filters (p-value <0.001). In addition, speedVac concentration using negatively charged filtration resulted in greater rotavirus recovery than that using positively charged filtration (p-value = 0.004). Thirty eight environmental water samples were collected from river, domestic sewage, canals receiving raw sewage drains, and tap water collected in containers for domestic use, all from congested areas of Bangkok. In addition, several samples of commercial drinking water were analyzed. All samples were concentrated and examined for rotavirus antigen. Coliforms and fecal coliforms (0->1,800 MPN/100 ml) were observed but rotavirus was not detected in any sample. This study suggests that the speedVac reconcentration method gives the most efficient rotavirus recovery from water samples.