Publication: Interaction of dengue virus envelope protein with endoplasmic reticulum-resident chaperones facilitates dengue virus production
Issued Date
2009-02-06
Resource Type
ISSN
10902104
0006291X
0006291X
Other identifier(s)
2-s2.0-58149529737
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.379, No.2 (2009), 196-200
Suggested Citation
Thawornchai Limjindaporn, Wiyada Wongwiwat, Sansanee Noisakran, Chatchawan Srisawat, Janjuree Netsawang, Chunya Puttikhunt, Watchara Kasinrerk, Panisadee Avirutnan, Somchai Thiemmeca, Rungtawan Sriburi, Nopporn Sittisombut, Prida Malasit, Pa thai Yenchitsomanus Interaction of dengue virus envelope protein with endoplasmic reticulum-resident chaperones facilitates dengue virus production. Biochemical and Biophysical Research Communications. Vol.379, No.2 (2009), 196-200. doi:10.1016/j.bbrc.2008.12.070 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27280
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Title
Interaction of dengue virus envelope protein with endoplasmic reticulum-resident chaperones facilitates dengue virus production
Abstract
Dengue virus infection is an important mosquito-borne disease and a public health problem worldwide. A better understanding of interactions between human cellular host and dengue virus proteins will provide insight into dengue virus replication and cellular pathogenesis. The glycosylated envelope protein of dengue virus, DENV E, is processed in the endoplasmic reticulum of host cells and therefore reliant on host processing functions. The complement of host ER functions involved and nature of the interactions with DENV E has not been thoroughly investigated. By employing a yeast two-hybrid assay, we found that domain III of DENV E interacts with human immunoglobulin heavy chain binding protein (BiP). The relevance of this interaction was demonstrated by co-immunoprecipitation and co-localization of BiP and DENV E in dengue virus-infected cells. Using the same approach, association of DENV E with two other chaperones, calnexin and calreticulin was also observed. Knocking-down expression of BiP, calnexin, or calreticulin by siRNA significantly decreased the production of infectious dengue virions. These results indicate that the interaction of these three chaperones with DENV E plays an important role in virion production, likely facilitating proper folding and assembly of dengue proteins. © 2008 Elsevier Inc. All rights reserved.