Publication:
Nationwide surveillance and molecular characterization of critically drug-resistant gram-negative bacteria: Results of the research university network Thailand study

Issued Date

2021-09-01

Resource Type

Article

File Type

video/youtube

ISSN

10986596
00664804

DOI

10.1128/AAC.00675-21

Other identifier(s)

2-s2.0-85112850482

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Antimicrobial Agents and Chemotherapy. Vol.65, No.9 (2021)

Suggested Citation

Thitiya Yungyuen, Tanittha Chatsuwan, Rongpong Plongla, Sakawrat Kanthawong, Umaporn Yordpratum, Supayang P. Voravuthikunchai, Sarunyou Chusri, Dennapa Saeloh, Worada Samosornsuk, Nuntra Suwantarat, Romanee Chaiwarith, Surat Wannalerdsakun, Porpon Rotjanapan, Prawat Chantharit, Orawan Tulyaprawat, Iyarit Thaipisuttikul, Pattarachai Kiratisin, Pakpiengpen Sornwiboonsak, Nanthawadee Thongnark, Niporn Thepchai, Pattraporn Ponglorpisit, Pimpa Rungnobhakhun, Wijittraporn Onraj, Piyanat Pitakwong, Watchanarat Nitchot, Jareerat Saensud, Jintana Phimsen, Theerasak Jaifoo, Nuttiya Srisurat, Krittika Kamlangharn, Napawan Punakabutra, Sirirat Lukin, Nalinwan Wongsawat, Supat Nantasan, Kanokwan Saengsawang, Thitirat Tangkosakul, Parichat Boonrod, Nattagarn Chuenchom, Thanee Wongchai, Phadungkiat Khamnoi, Khanuengnit Nuket, Wilawan Thipmontri, Athita Riwlord, Surachai Jirapornchai, Patcharee Runapong, Sutas Boonyong, Pongpol Rerknawarat, Uraiwan Surin, Poramed Winichakoon, Pisitphon Chaimongkhol, Sarunya Srijuntongsiri, Tanatporn Chuenkeson, Patsanporn Nontaratron, Palakorn Srinitiwat, Khoriah Chedee, Vichaya Yavichai, Nutchada Tanburawong, Jirayu Visuthranukul, Umnat Klamem, Nithiarphar Bamrungsri, Pathanith Aranya, Malee Techapornrung, Sasitorn Thaicharoen, Teerarat Shanthachol, Suwimol Chooprayoon, Sarobon Chaudakshetrin, Somjit Chinapak, Narisorn Lorchirachoonkul, Puncharat Wongut-Sa, Tidarat Suttiraksa, Pachara Kasemsiri, Waranya Srichunta, Sitthipong Dumrongpiwat, Chonteera Charoenchai, Wittaya Wangsomboonsiri, Kwanjai Ketwong, Sujinda Ruangchan, Benjamat Jareeyaphadub, Sarawut Promsen, Jiraporn Nilsakul, Sunee Chayangsu, Saowarat Deekae, Huttaya Thuncharoon, Patcharee Choochur, Narissara Mungkornkaew, Chitprasong Malaisri, Yaowares Panjakun, Adisak Sangchankoom, Sarochin Pangnakorn, Tananun Tanpaibule, Daranee Nutalai, Pannawadee Uppathamnarakorn, Warattaya Ueaphathanaphanich, Nutwariya Homsukon, Narumol Boonchaiyang Nationwide surveillance and molecular characterization of critically drug-resistant gram-negative bacteria: Results of the research university network Thailand study. Antimicrobial Agents and Chemotherapy. Vol.65, No.9 (2021). doi:10.1128/AAC.00675-21 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/77903

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Title

Nationwide surveillance and molecular characterization of critically drug-resistant gram-negative bacteria: Results of the research university network Thailand study

Author(s)

Thitiya Yungyuen
 Tanittha Chatsuwan
 Rongpong Plongla
 Sakawrat Kanthawong
 Umaporn Yordpratum
 Supayang P. Voravuthikunchai
 Sarunyou Chusri
 Dennapa Saeloh
 Worada Samosornsuk
 Nuntra Suwantarat
 Romanee Chaiwarith
 Surat Wannalerdsakun
 Porpon Rotjanapan
 Prawat Chantharit
 Orawan Tulyaprawat
 Iyarit Thaipisuttikul
 Pattarachai Kiratisin
 Pakpiengpen Sornwiboonsak
 Nanthawadee Thongnark
 Niporn Thepchai
 Pattraporn Ponglorpisit
 Pimpa Rungnobhakhun
 Wijittraporn Onraj
 Piyanat Pitakwong
 Watchanarat Nitchot
 Jareerat Saensud
 Jintana Phimsen
 Theerasak Jaifoo
 Nuttiya Srisurat
 Krittika Kamlangharn
 Napawan Punakabutra
 Sirirat Lukin
 Nalinwan Wongsawat
 Supat Nantasan
 Kanokwan Saengsawang
 Thitirat Tangkosakul
 Parichat Boonrod
 Nattagarn Chuenchom
 Thanee Wongchai
 Phadungkiat Khamnoi
 Khanuengnit Nuket
 Wilawan Thipmontri
 Athita Riwlord
 Surachai Jirapornchai
 Patcharee Runapong
 Sutas Boonyong
 Pongpol Rerknawarat
 Uraiwan Surin
 Poramed Winichakoon
 Pisitphon Chaimongkhol
 Sarunya Srijuntongsiri
 Tanatporn Chuenkeson
 Patsanporn Nontaratron
 Palakorn Srinitiwat
 Khoriah Chedee
 Vichaya Yavichai
 Nutchada Tanburawong
 Jirayu Visuthranukul
 Umnat Klamem
 Nithiarphar Bamrungsri
 Pathanith Aranya
 Malee Techapornrung
 Sasitorn Thaicharoen
 Teerarat Shanthachol
 Suwimol Chooprayoon
 Sarobon Chaudakshetrin
 Somjit Chinapak
 Narisorn Lorchirachoonkul
 Puncharat Wongut-Sa
 Tidarat Suttiraksa
 Pachara Kasemsiri
 Waranya Srichunta
 Sitthipong Dumrongpiwat
 Chonteera Charoenchai
 Wittaya Wangsomboonsiri
 Kwanjai Ketwong
 Sujinda Ruangchan
 Benjamat Jareeyaphadub
 Sarawut Promsen
 Jiraporn Nilsakul
 Sunee Chayangsu
 Saowarat Deekae
 Huttaya Thuncharoon
 Patcharee Choochur
 Narissara Mungkornkaew
 Chitprasong Malaisri
 Yaowares Panjakun
 Adisak Sangchankoom
 Sarochin Pangnakorn
 Tananun Tanpaibule
 Daranee Nutalai
 Pannawadee Uppathamnarakorn
 Warattaya Ueaphathanaphanich
 Nutwariya Homsukon
 Narumol Boonchaiyang

Other Contributor(s)

Ramathibodi Hospital
 Siriraj Hospital
 Sukhothai Hospital
 Udon Thani Center Hospital
 Nakornping Hospital
 Thammasat University Hospital
 Lampang Hospital
 Ban Pong Hospital
 Prapokklao Hospital
 Chulalongkorn University
 Hatyai Hospital
 Songkhla Hospital
 Faculty of Medicine, Khon Kaen University
 Faculty of Medicine, Prince of Songkia University
 Trang Hospital
 Police General Hospital
 Bhumibol Adulyadej Hospital
 Lerdsin Hospital
 Surin Hospital
 Naresuan University
 King Chulalongkorn Memorial Hospital
 Kamphaeng Phet Provincial Hospital
 Vajira Hospital
 Taksin Hospital
 Maharaj Nakorn Chiang Mai Hospital
 Thammasat University
 Vichaiyut Hospital
 Prince of Songkla University
 Sawanpracharak Hospital
 Chiang Mai University
 Ratchaburi Regional Hospital
 Saraburi Hospital
 Wichianburi Hospital
 Khon Kean Hospital
 Mongkutwattana Hospital
 Maharat Nakhon Ratchasima Hospital
 Nakhon Pathom Hospital
 Bueng Kan Hospital
 Sakonnakhon Hospital
 Queen Savang Vadhana Memorial Hospital
 Maesot Hospital
 King Narai Hospital
 Angthong Hospital
 Sunpasitthiprasong Hospital
 Nakhon Phanom Provincial Hospital
 Chum Phae Hospital
 Phayao Hospital
 Panyananthaphikkhu Chonprathan Medical Center
 Pranangklao Hospital
 Roi Et Hospital
 Sawang Dandin Crown Prince Hospital
 Ramkhamhaeng Hospital

Abstract

A large-scale surveillance is an important measure to monitor the regional spread of antimicrobial resistance. We prospectively studied the prevalence and molecular characteristics of clinically important Gram-negative bacilli, including Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii complex (ABC), and Pseudomonas aeruginosa, from blood, respiratory tract, urine, and sterile sites at 47 hospitals across Thailand. Among 187, 619 isolates, 93, 810 isolates (50.0%) were critically drug resistant, of which 12, 915 isolates (13.8%) were randomly selected for molecular characterization. E. coli was most commonly isolated from all specimens, except the respiratory tract, in which ABC was predominant. Prevalence of extendedspectrum cephalosporin resistance (ESCR) was higher in E. coli (42.5%) than K. pneumoniae (32.0%), but carbapenem-resistant (CR)-K. pneumoniae (17.2%) was 4.5-fold higher than CR-E. coli (3.8%). The majority of ESCR/CR-E. coli and K. pneumoniae isolates carried blaCTX-M (64.6% to 82.1%). BlaNDM and blaOXA-48-like were the most prevalent carbapenemase genes in CR-E. coli/CR-K. pneumoniae (74.9%/52.9% and 22.4%/ 54.1%, respectively). In addition, 12.9%/23.0% of CR-E. coli/CR-K. pneumoniae cocarried blaNDM and blaOXA-48-like. Among ABC isolates, 41.9% were extensively drug resistant (XDR) and 35.7% were multidrug resistant (MDR), while P. aeruginosa showed XDR/MDR at 6.3%/16.5%. A. baumannii was the most common species among ABC isolates. The major carbapenemase gene in MDR-A. baumannii/XDR-A. baumannii was blaOXA-23-like (85.8%/93.0%), which had much higher rates than other ABC species. BlaIMP, blaVIM, blaOXA-40-like, and blaOXA-58-like were also detected in ABC at lower rates. The most common carbapenemase gene in MDR/XDR-P. aeruginosa was blaIMP (29.0%/30.6%), followed by blaVIM (9.5%/25.3%). The findings reiterate an alarming situation of drug resistance that requires serious control measures.

Keyword(s)

Medicine
 Pharmacology, Toxicology and Pharmaceutics

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/77903

Collections

Scopus 2021