Publication: Third-stage Gnathostoma spinigerum larva excretory secretory antigens modulate function of Fc gamma receptor I-mediated monocytes in peripheral blood mononuclear cell culture
Issued Date
2016
Valid Date
2017-11-10
Resource Type
Language
eng
Rights
Mahidol University
Rights Holder(s)
BioMed Central
Bibliographic Citation
Tropical Medicine and Health. Vol.44, (2016), 5
Suggested Citation
Surachet Benjathummarak, Ratchanok Kumsiri, Supaporn Nuamtanong, Thareerat Kalambaheti, Jitra Waikagul, Nareerat Viseshakul, Yaowapa Maneerat Third-stage Gnathostoma spinigerum larva excretory secretory antigens modulate function of Fc gamma receptor I-mediated monocytes in peripheral blood mononuclear cell culture. Tropical Medicine and Health. Vol.44, (2016), 5. doi:10.1186/s41182-016-0005-x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/3102
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Third-stage Gnathostoma spinigerum larva excretory secretory antigens modulate function of Fc gamma receptor I-mediated monocytes in peripheral blood mononuclear cell culture
Other Contributor(s)
Abstract
Background: Third (infective)-stage Gnathostoma spinigerum larvae (L3) mainly cause human gnathostomiasis. G.
spinigerum L3 migrate throughout the subcutaneous tissues, vital organs, and central nervous system and can cause
various pathogenesis including sudden death. Interestingly, G. spinigerum L3 can survive and evade host cellular
immunity for months or years. The effects of G. spinigerum excretory-secretory (ES) products involved in larval migration
and immune-evasive strategies are unknown. Monocytes are innate immune cells that act as phagocytic and antigenpresenting
cells and also play roles against helminthic infections via a complex interplay between other immune cells.
Fc gamma receptor I (FcγRI) is a high-affinity receptor that is particularly expressed on monocytes, macrophages, and
dendritic cells. The cross-linking of FcγRI and antigen-antibody complex initiates signal transduction cascades in
phagocytosis, cytokine production, and antibody-dependent cell-mediated cytotoxicity (ADCC). This study investigated
whether ES antigen (ESA) from G. spinigerum L3 affects monocyte functions.
Results: Cultures of normal peripheral blood mononuclear cells (PBMC) separated from healthy buffy coats were used
as a human immune cell model. ESA was prepared from G. spinigerum L3 culture. Using Real-Time quantitative reverse
transcription-polymerase chain reaction (qRT-PCR), the effect of ESA to down-regulate FcγRI mRNA expression in
monocytes during 90 min of observation was not well delineated. Flow cytometry analysis revealed a significant
phenotypic-decreased FcγRI expression on the monocyte surface at 12 hours (h) of cultivation with the ESA (p = 0.033).
Significantly reduced monocyte-mediated phagocytosis capacity was consistently observed after 12 h of ESA
pretreatment (p = 0.001).
Conclusions: Our results suggest that G. spinigerum ESA modulates monocyte function via depletion of FcγRI
expression. This study provides preliminary information for future in-depth studies to elucidate mechanisms of
the immune-evasive strategy of G. spinigerum larvae.